caa a22 4500 606218742 CHVBK 20210128101114.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11104-015-2389-5 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2389-5 Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere [Elektronische Daten] [Paula Garcia-Fraile, Jonathan Seaman, Ramakrishnan Karunakaran, Anne Edwards, Philip Poole, J. Downie] Background and aims: To form nitrogen-fixing nodules on pea roots, Rhizobium leguminosarum biovar viciae must be competitive in the rhizosphere. Our aim was to identify genes important for rhizosphere fitness. Methods: Signature-tagged mutants were screened using microarrays to identify mutants reduced for growth in pea rhizospheres. Candidate mutants were assessed relative to controls for growth in minimal medium, growth in pea rhizospheres and for infection of peas in mixed inoculants. Mutated genes were identified by DNA sequencing and confirmed by transduction. Results: Of 5508 signature-tagged mutants, microarrays implicated 50 as having decreased rhizosphere fitness. Growth tests identified six mutants with rhizosphere-specific phenotypes. The mutation in one of the genes (araE) was in an arabinose catabolism operon and blocked growth on arabinose. The mutation in another gene (pcaM), encoding a predicted solute binding protein for protocatechuate and hydroxybenzoate uptake, decreased growth on protocatechuate. Both mutants were decreased for nodule infection competitiveness with mixed inoculants, but nodulated peas normally when inoculated alone. Other mutants with similar phenotypes had mutations predicted to affect secondary metabolism. Conclusions: Catabolism of arabinose and protocatechuate in the pea rhizosphere is important for competitiveness of R.l. viciae. Other genes predicted to be involved in secondary metabolism are also important. The Author(s), 2015 Competitive nodule infection nationallicence Legume nodulation nationallicence Pisum nationallicence Rhizosphere fitness nationallicence Garcia-Fraile Paula Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut Seaman Jonathan School of Biological Sciences, University of Reading, RG6 6AJ, Reading, UK aut Karunakaran Ramakrishnan Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut Edwards Anne Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut Poole Philip Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut Downie J. Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut Plant and Soil Springer International Publishing 390/1-2(2015-05-01), 251-264 0032-079X 390:1-2<251 2015 390 11104 https://doi.org/10.1007/s11104-015-2389-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2389-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Garcia-Fraile Paula Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut NATIONALLICENCE 700 1- Seaman Jonathan School of Biological Sciences, University of Reading, RG6 6AJ, Reading, UK aut NATIONALLICENCE 700 1- Karunakaran Ramakrishnan Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut NATIONALLICENCE 700 1- Edwards Anne Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut NATIONALLICENCE 700 1- Poole Philip Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut NATIONALLICENCE 700 1- Downie J. Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 390/1-2(2015-05-01), 251-264 0032-079X 390:1-2<251 2015 390 11104