caa a22 4500 606218750 CHVBK 20210128101114.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11104-014-2375-3 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2375-3 Linking root traits to copper exclusion mechanisms in Silene paradoxa L. (Caryophyllaceae) [Elektronische Daten] [Ilaria Colzi, Sara Pignattelli, Elisabetta Giorni, Alessio Papini, Cristina Gonnelli] Background and aims: Copper is one of the most important pollutants in mine-contaminated soils. This study tests the response in a sensitive population vs a tolerant one of the model species Silene paradoxa in order to understand the general mechanisms of tolerance at the micromorphological and ultrastructural level. Methods: Two populations of Silene paradoxa were grown in hydroponics and exposed to different CuSO4 treatments. The roots were investigated with light, fluorescence and transmission electron microscope. Callose and lignin were spectrophotometrically determined. Results: The tolerant population constitutively possessed a higher amount of mucilage and was able to reduce the length of the zone between the apex and the first lignified tracheids. Callose production decreased. It did not show remarkable copper-induced ultrastructural modifications, apart from the presence of precipitates in the tangential walls. The sensitive population showed huge nucleoli with a spongy periphery in the central cylinder together with the presence of electrondense granules in the mitochondria. Plastids were rarely observed and generally very electrondense and elongated. Conclusions: n the copper tolerant population of S. paradoxa some of the root traits concurring to generate metal-excluding roots were suggested to be mucilage and lignin production and the reduction of the subapical root zone. Springer International Publishing Switzerland, 2015 Root nationallicence Copper exclusion nationallicence Lignin nationallicence Callose nationallicence Tolerance to copper nationallicence Silene paradoxa nationallicence Colzi Ilaria Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut Pignattelli Sara Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut Giorni Elisabetta Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut Papini Alessio Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut Gonnelli Cristina Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut Plant and Soil Springer International Publishing 390/1-2(2015-05-01), 1-15 0032-079X 390:1-2<1 2015 390 11104 https://doi.org/10.1007/s11104-014-2375-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2375-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Colzi Ilaria Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut NATIONALLICENCE 700 1- Pignattelli Sara Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut NATIONALLICENCE 700 1- Giorni Elisabetta Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut NATIONALLICENCE 700 1- Papini Alessio Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut NATIONALLICENCE 700 1- Gonnelli Cristina Department of Biology, Università di Firenze, via Micheli 1-3, 50121, Florence, Italy aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 390/1-2(2015-05-01), 1-15 0032-079X 390:1-2<1 2015 390 11104