caa a22 4500 606218777 CHVBK 20210128101114.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11104-015-2396-6 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2396-6 Physiological mechanisms of adaptation of Dianthus carthusianorum L. to growth on a Zn-Pb waste deposit-the case of chronic multi-metal and acute Zn stress [Elektronische Daten] [Małgorzata Wójcik, Sławomir Dresler, Anna Tukiendorf] Aims: This study investigates the response of metallicolous (M) and nonmetallicolous (NM) ecotypes of Dianthus carthusianorum L. to chronic multi-metal and acute Zn stress. Methods: Plants were cultivated on the Zn-Pb waste heap substrate and under Zn excess in hydroponics. Growth parameters as well as accumulation of organic acids and thiol peptides were determined as a function of metal accumulation. Results: When grown on the metalliferous substrate, the M plants showed less phytotoxicity symptoms, lower foliar metal (Zn, Pb, Cd) accumulation, higher malate and citrate but lower glutathione content than the NM plants. When exposed to Zn excess in hydroponics, the M ecotype was also more tolerant but accumulated more Zn in comparison with the NM ecotype, accompanied by greater malate and citrate concentrations in the shoots, which were however not affected by increasing Zn doses. No phytochelatins were detected under any experimental conditions. Conclusions: Both constitutive and adaptive tolerance was found in D. carthusianorum. Under chronic metal stress, enhanced tolerance results from restricted metal uptake to the shoots and probably from detoxification by organic acids; however, under acute Zn stress it is not related to diminished metal uptake or organic acids. Glutathione and phytochelatins are not implicated in adaptive metal tolerance. The Author(s), 2015 Adaptive and constitutive tolerance nationallicence Glutathione nationallicence Organic acids nationallicence Pseudometallophyte nationallicence Zn tolerance nationallicence Wójcik Małgorzata Department of Plant Physiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, PL-20-033, Lublin, Poland aut Dresler Sławomir Department of Plant Physiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, PL-20-033, Lublin, Poland aut Tukiendorf Anna Department of Plant Physiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, PL-20-033, Lublin, Poland aut Plant and Soil Springer International Publishing 390/1-2(2015-05-01), 237-250 0032-079X 390:1-2<237 2015 390 11104 https://doi.org/10.1007/s11104-015-2396-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2396-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wójcik Małgorzata Department of Plant Physiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, PL-20-033, Lublin, Poland aut NATIONALLICENCE 700 1- Dresler Sławomir Department of Plant Physiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, PL-20-033, Lublin, Poland aut NATIONALLICENCE 700 1- Tukiendorf Anna Department of Plant Physiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, PL-20-033, Lublin, Poland aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 390/1-2(2015-05-01), 237-250 0032-079X 390:1-2<237 2015 390 11104