caa a22 4500 606218912 CHVBK 20210128101115.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11104-015-2397-5 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2397-5 Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley [Elektronische Daten] [Aster Alemayehu, Veronika Zelinová, Beáta Bočová, Jana Huttová, Igor Mistrík, Ladislav Tamás] Background and Aims: The objective of this study was to analyse the role of nitric oxide (NO) in relation to reactive oxygen species (ROS) production and root growth inhibition in the early response of barley root tip to cadmium. Methods: We used cell-permeable NO-binding dye DAF-2DA for the NO localization and cell-impermeable dye DAR-4M for the quantification of NO in barley root apex. Results: A considerable increase in NO production was detected in the transition zone of root tips already 30min after the immersion of barley roots into 15μM Cd. This Cd-induced NO generation further increased during the recovery period and reached its maximum level 2h after the short-term treatment. At this stage an enhanced Cd-induced NO generation was observed in the meristematic, transition and in the beginning of elongation zone in comparison with control roots. Inhibitor of NO synthase activity markedly reduced root growth in control seedlings and had a synergistic growth inhibitory effect with Cd. On the contrary, this Cd-induced root growth inhibition was markedly alleviated by the NO donor. Exposure of roots to Cd markedly increased the production of ROS in the proximal elongation zone of root. While NO did not affect ROS generation, inhibition of its synthesis slightly decreased ROS generation in both control and Cd-treated roots. Conclusions: Our results revealed that enhanced NO production is a very early response of barley root to Cd stress and it is involved in the regulation of root growth mitigating Cd-induced root growth inhibition. Springer International Publishing Switzerland, 2015 Barley nationallicence Cadmium nationallicence Nitric oxide nationallicence Reactive oxygen species nationallicence Root growth inhibition nationallicence DAF-2DA : 4,5-Diaminofluorescein diacetate nationallicence DAR-4M : Diaminorhodamine-4M nationallicence NO : Nitric oxide nationallicence L-NAME : L-Nω-nitroarginine methyl ester nationallicence PFC : Potassium ferricyanide nationallicence ROS : Reactive oxygen species nationallicence SNP : Sodium nitroprusside nationallicence Alemayehu Aster Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut Zelinová Veronika Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut Bočová Beáta Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut Huttová Jana Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut Mistrík Igor Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut Tamás Ladislav Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut Plant and Soil Springer International Publishing 390/1-2(2015-05-01), 213-222 0032-079X 390:1-2<213 2015 390 11104 https://doi.org/10.1007/s11104-015-2397-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2397-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alemayehu Aster Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Zelinová Veronika Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Bočová Beáta Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Huttová Jana Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Mistrík Igor Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Tamás Ladislav Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 390/1-2(2015-05-01), 213-222 0032-079X 390:1-2<213 2015 390 11104