caa a22 4500 60621898X CHVBK 20210128101115.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11104-015-2431-7 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2431-7 Above- and belowground linkages of a nitrogen and phosphorus co-limited tropical mountain pasture system - responses to nutrient enrichment [Elektronische Daten] [Alexander Tischer, Martin Werisch, Franziska Döbbelin, Tessa Camenzind, Matthias Rillig, Karin Potthast, Ute Hamer] Aim: Little is known about how N and P co-limited ecosystems respond to single nutrient enrichment. This work assesses the susceptibility of above- and belowground ecosystem components and of their linkages in an N and P co-limited pasture to N- and P-enrichment. We tested if the plants' responses can be explained by the concept of serially linked nutrients introduced by Ågren (Ecol Lett 7:185-191, 2004). In this concept, the control of the growth rate by one nutrient is assumed to depend on the control of a different cellular process by another nutrient. Methods: We investigated the responses of shoot and root biomass and C:N:P stoichiometry of the grass Setaria sphacelata (Schumach.) to moderate N, P, and N + P application over 5years. In addition, the effects of nutrient enrichment on soil nutrient pools, on arbuscular mycorrhizal fungi (AMF) as well as on microbial biomass, activity, and community structure (phospholipid fatty acids: PLFA) were tested. In order to evaluate the importance of different factors explaining microbial responses, we applied a likelihood-based information-theoretic approach. Results: The application of N + P increased aboveground grass biomass (+61%). Root biomass was stimulated by P-treatment (+45%). Grass C:N:P stoichiometry responded by altering the P-uptake (P-treatment) or by translocating P from shoot to root (N-treatment). In particular, root C:N and C:P stoichiometry decreased in P- and in N-treatment. Extractable fractions of soil C, N, and P were significantly affected by nutrient enrichment. P application increased the biomass of Gram-positive bacteria (+22%) and the abundance of AMF (+46%), however, results of the IT-approach suggested indirect effects of nutrient enrichment on microbes. Conclusions: The responses of the N and P co-limited pasture to particular nutrient enrichment support the concept of serially linked nutrients. The present study provides evidence for the fundamental importance of P for controlling resource allocation of plants in responses to nutrient enrichment. Resource allocation of the grass rather than direct effects of nutrient additions drives changes in AMF, microbial biomass, community structure, and activity. Springer International Publishing Switzerland, 2015 Arbuscular mycorrhizal fungi nationallicence C:N:P stoichiometry nationallicence Dissolved organic carbon nationallicence Information-theoretic approach nationallicence Soil microbial biomass nationallicence Soil microbial community structure nationallicence Tischer Alexander Institute of Soil Science and Site Ecology, Dresden University of Technology, Pienner Straße 19, 01737, Tharandt, Germany aut Werisch Martin Institute of Forest Growth and Computer Sciences, Dresden University of Technology, Pienner Straße 8, 01737, Tharandt, Germany aut Döbbelin Franziska Institute of Biology, Freie Universität Berlin, Altensteinstraße 6, 14195, Berlin, Germany aut Camenzind Tessa Institute of Biology, Freie Universität Berlin, Altensteinstraße 6, 14195, Berlin, Germany aut Rillig Matthias Institute of Biology, Freie Universität Berlin, Altensteinstraße 6, 14195, Berlin, Germany aut Potthast Karin Institute of Soil Science and Site Ecology, Dresden University of Technology, Pienner Straße 19, 01737, Tharandt, Germany aut Hamer Ute Institute of Soil Science and Site Ecology, Dresden University of Technology, Pienner Straße 19, 01737, Tharandt, Germany aut Plant and Soil Springer International Publishing 391/1-2(2015-06-01), 333-352 0032-079X 391:1-2<333 2015 391 11104 https://doi.org/10.1007/s11104-015-2431-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2431-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tischer Alexander Institute of Soil Science and Site Ecology, Dresden University of Technology, Pienner Straße 19, 01737, Tharandt, Germany aut NATIONALLICENCE 700 1- Werisch Martin Institute of Forest Growth and Computer Sciences, Dresden University of Technology, Pienner Straße 8, 01737, Tharandt, Germany aut NATIONALLICENCE 700 1- Döbbelin Franziska Institute of Biology, Freie Universität Berlin, Altensteinstraße 6, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Camenzind Tessa Institute of Biology, Freie Universität Berlin, Altensteinstraße 6, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Rillig Matthias Institute of Biology, Freie Universität Berlin, Altensteinstraße 6, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Potthast Karin Institute of Soil Science and Site Ecology, Dresden University of Technology, Pienner Straße 19, 01737, Tharandt, Germany aut NATIONALLICENCE 700 1- Hamer Ute Institute of Soil Science and Site Ecology, Dresden University of Technology, Pienner Straße 19, 01737, Tharandt, Germany aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 391/1-2(2015-06-01), 333-352 0032-079X 391:1-2<333 2015 391 11104