caa a22 4500 606218998 CHVBK 20210128101115.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11104-015-2395-7 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2395-7 Genotypic differences in antioxidant response to phosphorus deficiency in Brassica napus [Elektronische Daten] [Shuisen Chen, Hua Zhao, Guangda Ding, Fangsen Xu] Background and aims: The effect of phosphorus (P) deficiency on plant growth and development depends on the ability of plants to adapt to induced stress. Oxidative stress may play a vital role in P deficient Brassica napus and the antioxidant ability of this plant may be an important mechanism for adapting to P deficiency. The present study aims to better understand the correlation between high P use efficiency and antioxidative ability by comparing the activity of antioxidant enzymes and lipid peroxidation in the leaves and roots of two B. napus genotypes. Methods: The response of the antioxidant defense system of two B. napus genotypes with different P use efficiencies to short-term P-free starvation and long-term low P stress were investigated by analyzing the activities of antioxidative enzymes, the superoxide free radical ion (O2 ·−) and H2O2 concentration, and the level of lipid peroxidation. Results: The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), and glutathione reductase (GR) in the leaves and roots were increased in both genotypes under the two P treatments. However, the activities of some of these enzymes exhibited tissue or genotype specificity. In the P use-inefficient genotype "B104-2,” more O2 ·− was accumulated and a higher level of lipid peroxidation was induced under P deficiency conditions compared with P use-efficient genotype "Eyou Changjia.” The GPX activity in the roots was considerably higher (20- to 30-fold) than in the leaves, whereas the CAT activity in the roots was lower (approximately 3-fold) than in the leaves in both genotypes. Conclusions: Compared with P use-inefficient genotype "B104-2,” P use-efficient genotype "Eyou Changjia” suffered less oxidative damage and lipid peroxidation, and inhibited O2 ·− generation more effectively under low P stress. Severe oxidative damage was accompanied with a higher antioxidative enzyme activities in "B104-2” under low P stress. Springer International Publishing Switzerland, 2015 P deficiency nationallicence Oxidative stress nationallicence Antioxidative enzymes nationallicence Lipid peroxidation nationallicence Brassica napus nationallicence Genotypic difference nationallicence Chen Shuisen National Key Laboratory of Crop Genetic Improvement and Microelement Research Center, Huazhong Agricultural University, 430070, Wuhan, China aut Zhao Hua National Key Laboratory of Crop Genetic Improvement and Microelement Research Center, Huazhong Agricultural University, 430070, Wuhan, China aut Ding Guangda National Key Laboratory of Crop Genetic Improvement and Microelement Research Center, Huazhong Agricultural University, 430070, Wuhan, China aut Xu Fangsen National Key Laboratory of Crop Genetic Improvement and Microelement Research Center, Huazhong Agricultural University, 430070, Wuhan, China aut Plant and Soil Springer International Publishing 391/1-2(2015-06-01), 19-32 0032-079X 391:1-2<19 2015 391 11104 https://doi.org/10.1007/s11104-015-2395-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2395-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Shuisen National Key Laboratory of Crop Genetic Improvement and Microelement Research Center, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Zhao Hua National Key Laboratory of Crop Genetic Improvement and Microelement Research Center, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Ding Guangda National Key Laboratory of Crop Genetic Improvement and Microelement Research Center, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Xu Fangsen National Key Laboratory of Crop Genetic Improvement and Microelement Research Center, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 391/1-2(2015-06-01), 19-32 0032-079X 391:1-2<19 2015 391 11104