caa a22 4500 606219102 CHVBK 20210128101116.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11104-015-2405-9 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2405-9 Differential physiological responses in rice upon exposure to excess distinct iron forms [Elektronische Daten] [Caroline Müller, Kacilda Kuki, Daniel Pinheiro, Laíse de Souza, Advânio Siqueira Silva, Marcelo Loureiro, Marco Oliva, Andréa Almeida] Background and aims: Rice can be cultivated in highlands, which can expose it to iron deficiency, or under irrigation, which can lead to iron toxicity and lower productivity. This study aimed to investigate the strategies used by rice plants under different divalent and trivalent sources of iron excess. Methods: Rice plants from a lowland and upland cultivar were grown in nutrient solution with toxic concentrations of ferrous or ferric iron. A mineral nutrient quantification and anatomical analysis were performed on leaves and roots. Physiological damage was assessed by leaf photochemical parameters and lipid peroxidation. Expression levels of genes related to iron homeostasis were analyzed. Results: More pronounced nutritional deficiencies, oxidative stress and physiological damage were observed in plants exposed to toxic levels of ferrous iron. Ferritin expression increased in leaves of both cultivars under ferrous or ferric iron excess. Conclusions: We showed that sulfate iron was more toxic to the two rice cultivars even though this iron source was less translocated in the plant. Trivalent iron complexed to citrate is easily translocated through rice plants, but it is less toxic than the divalent iron. Rice plants are able to cope with this iron overload by keeping photosynthetic apparatus working properly. The Author(s), 2015 Oryza sativa nationallicence Photochemical reactions nationallicence Mineral nutrition nationallicence Gene expression nationallicence Iron toxicity nationallicence EDTA : Ethylenediamine tetraacetic acid nationallicence MDA : Malondialdehyde nationallicence PSII : Photosystem II nationallicence F 0 : Minimal chlorophyll fluorescence nationallicence F v/ F m : Maximal photochemical efficiency of PSII nationallicence YII : Quantum yield of photochemical energy conversion in PSII nationallicence qRT-PCR : Quantitative reverse transcriptase polymerase chain reaction nationallicence FER1 : Ferritin nationallicence PSBA : Chloroplastic protein Q-B that encodes PSII reaction center protein D1 nationallicence GS1 : Cytosolic glutamine synthetase nationallicence NAS2 : Nicotianamine synthetase 2 nationallicence YSL1 : Yellow stripe-like 1 nationallicence PI1 : Phosphate-limitation I nducible Gene 1, a tonoplast monosaccharide transporter inducible by phosphate 1 limitation nationallicence PTF1 : Inducible bHLH by phosphate 1 limitation transcription factor nationallicence Müller Caroline Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut Kuki Kacilda Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut Pinheiro Daniel Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut de Souza Laíse Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut Siqueira Silva Advânio Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut Loureiro Marcelo Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut Oliva Marco Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut Almeida Andréa Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut Plant and Soil Springer International Publishing 391/1-2(2015-06-01), 123-138 0032-079X 391:1-2<123 2015 391 11104 https://doi.org/10.1007/s11104-015-2405-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2405-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Müller Caroline Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Kuki Kacilda Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Pinheiro Daniel Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- de Souza Laíse Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Siqueira Silva Advânio Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Loureiro Marcelo Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Oliva Marco Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Almeida Andréa Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 391/1-2(2015-06-01), 123-138 0032-079X 391:1-2<123 2015 391 11104