caa a22 4500 606219250 CHVBK 20210128101117.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11104-015-2399-3 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2399-3 The response of root-associated bacterial community to the grafting of watermelon [Elektronische Daten] [Ning Ling, Yang Song, Waseem Raza, Qiwei Huang, Shiwei Guo, Qirong Shen] Background and aims: Grafting is commonly used to overcome soil-borne disease but how it affects the root-associated microbiome of concerned crops and the linkage between the microbiome and the resistance of soil-borne disease remain unknown. This study addressed the variation in the microbial activity and bacterial community in the rhizosphere of own-root bottle gourd (rootstock), own-root watermelon, and grafted-root watermelon under field conditions and tried to clarify how bacterial communities in the rhizosphere responded to grafting of watermelon. Methods: Seven types of soil enzyme activities were tested by microplate fluorometric assay, and the root-associated bacterial community was compared using 454 pyrosequencing. Results: Clear distinctions in microbial activity and taxonomic levels between the different treatments were obtained. Compared with grafted-root watermelon, ungrafted watermelon recruited significantly higher beneficial bacterial genera, such as Bacillus spp. and Paenibacillus spp., suggesting the grafted watermelon root could not have the ability to harbor highly beneficial bacteria to exert soil-borne disease resistance. However, a significantly higher Shannon-Wiener index at any reads level was found in the rhizosphere of grafted watermelon compared with ungrafted watermelon. Conclusions: Root-associated bacteria of grafted watermelon possess a broader niche overlap which would provide the potential to exclude the pathogen challenge. We proposed that the grafted watermelon might exert soil-borne disease resistance by maximizing the niche occupancy of rhizosphere rather than by recruiting more beneficial bacteria. Springer International Publishing Switzerland, 2015 Grafting nationallicence Watermelon nationallicence Soil enzyme nationallicence Bacterial community nationallicence 454 Pyrosequencing nationallicence Ling Ning Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut Song Yang Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut Raza Waseem Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut Huang Qiwei Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut Guo Shiwei Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut Shen Qirong Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut Plant and Soil Springer International Publishing 391/1-2(2015-06-01), 253-264 0032-079X 391:1-2<253 2015 391 11104 https://doi.org/10.1007/s11104-015-2399-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2399-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ling Ning Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Song Yang Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Raza Waseem Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Huang Qiwei Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Guo Shiwei Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Shen Qirong Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 391/1-2(2015-06-01), 253-264 0032-079X 391:1-2<253 2015 391 11104