caa a22 4500 606219293 CHVBK 20210128101117.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11104-015-2524-3 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2524-3 A humic substances product extracted from biochar reduces Arabidopsis root hair density and length under P-sufficient and P-starvation conditions [Elektronische Daten] [E. Graber, L. Tsechansky, E. Mayzlish-Gati, R. Shema, H. Koltai] Background and aims: Biochar additions may have positive impacts on phosphorus (P) availability to plants and cause down-regulation of genes associated with P starvation. Various alkaline-extracted humic substances products (HSP) also induce partial relief in plantsfrom P starvation and, moreover, cause an increase in total cell phosphate, ATP and glucose-6-phosphate levels. As many biochars contain substances similar in structure and functioning to HSP, our goal was to examine if such products extracted from biochar (B-HSP) could affect plant responses to initial P concentration (Pi) under Pi sufficient and starvation conditions. Methods: We examined the impact of B-HSP in the growing media of Arabidopsis seedlings on root hair development (length and density) in sterile systems, and evaluated whether nutrient complexation with B-HSP could account for observed differences. Results: Root hair length was significantly lower in B-HSP amended Pi-sufficient growing media, and root hair density was significantly lower in both B-HSP amended Pi sufficient and starvation regimes as compared with non-amended treatments. The differences did not result from either primary (P source) or secondary (increased P availability) nutritional effects. Conclusions: B-HSP appears to cause a change in plant perception of P nutrition. This may be another means by which biochar impacts growing plants. Springer International Publishing Switzerland, 2015 Root hair nationallicence Phosphorus nationallicence Biochar nationallicence Plant hormone nationallicence Humic acid nationallicence Humic acid products nationallicence Alkaline extract nationallicence Humic substances nationallicence P deficiency nationallicence Fulvic acid nationallicence Graber E. Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut Tsechansky L. Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut Mayzlish-Gati E. Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut Shema R. Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut Koltai H. Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 21-30 0032-079X 395:1-2<21 2015 395 11104 https://doi.org/10.1007/s11104-015-2524-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2524-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Graber E. Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Tsechansky L. Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Mayzlish-Gati E. Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Shema R. Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Koltai H. Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, POB 6, 50250, Bet Dagan, Israel aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 21-30 0032-079X 395:1-2<21 2015 395 11104