caa a22 4500 606219307 CHVBK 20210128101117.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11104-015-2557-7 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2557-7 Study of nitrogen and carbon transfer from soil organic matter to Tuber melanosporum mycorrhizas and ascocarps using 15N and 13C soil labelling and whole-genome oligoarrays [Elektronische Daten] [François Le Tacon, Bernd Zeller, Caroline Plain, Christian Hossann, Claude Bréchet, Francis Martin, Annegret Kohler, Jean Villerd, Christophe Robin] Background and aims: We previously showed by 13CO2 host labelling that almost all of the constitutive carbon allocated to the truffles originated from the host. The objective of this present work was to determine the putative capacity of T. melanosporum ectomycorrhizas and ascocarps to use soil carbon and to uptake or assimilate soil nitrate. Methods: The current investigation involved 13C and 15N soil labelling by incorporating labelled leaf litter and expression of genes involved in carbon and nitrogen metabolism in ascocarps and ectomycorrhizas. Results: The ascocarps harvested in the labelled plots were highly enriched in 15N but were almost never enriched in 13C. The main source of soil mineral nitrogen was nitrate. A nitrate transporter, one nitrate reductase and a nitrite reductase were well expressed in ectomycorrhizas. Several genes involved in aminoacid synthesis or in transamination processes were also well expressed in ectomycorrhizas. No nitrate transporter was expressed in ascocarps where the CAZyme genes upregulated were mainly Glycosyltransferases involved in saccharide transfer. Conclusion: Ascocarps did not exhibit saprotrophic capacity for C, supporting previous results from 13CO2 host labelling showing that C is provided by the host tree. The 15N present in the ascocarps after soil labelling is supplied as ammonium or aminoacids by the ectomycorrhizas, which are able to uptake, reduce and metabolize nitrate. Springer International Publishing Switzerland, 2015 Truffle nationallicence 13C nationallicence 15N nationallicence Ectomycorrhizas nationallicence Ascocarps nationallicence Gene expression nationallicence Le Tacon François INRA, Centre de Nancy Lorraine, UMR 1136, Interactions Arbres/Microrganismes (IAM), Labex ARBRE, 54280, Champenoux, France aut Zeller Bernd INRA, Centre de Nancy Lorraine, UR 1138, Biogéochimie des Ecosystèmes Forestiers (BEF), Labex ARBRE, 54280, Champenoux, France aut Plain Caroline INRA, Centre de Nancy Lorraine, UMR 1137, Ecologie et Ecophysiologie Forestières, Labex ARBRE, 54280, Champenoux, France aut Hossann Christian INRA, Centre de Nancy Lorraine, UMR 1137, Ecologie et Ecophysiologie Forestières, Labex ARBRE, 54280, Champenoux, France aut Bréchet Claude INRA, Centre de Nancy Lorraine, UMR 1137, Ecologie et Ecophysiologie Forestières, Labex ARBRE, 54280, Champenoux, France aut Martin Francis INRA, Centre de Nancy Lorraine, UMR 1136, Interactions Arbres/Microrganismes (IAM), Labex ARBRE, 54280, Champenoux, France aut Kohler Annegret INRA, Centre de Nancy Lorraine, UMR 1136, Interactions Arbres/Microrganismes (IAM), Labex ARBRE, 54280, Champenoux, France aut Villerd Jean Université de Lorraine, UMR 1121 « Agronomie & Environnement » Nancy-Colmar, F-54518, Vandoeuvre-les-Nancy, France aut Robin Christophe Université de Lorraine, UMR 1121 « Agronomie & Environnement » Nancy-Colmar, F-54518, Vandoeuvre-les-Nancy, France aut Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 351-373 0032-079X 395:1-2<351 2015 395 11104 https://doi.org/10.1007/s11104-015-2557-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2557-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Le Tacon François INRA, Centre de Nancy Lorraine, UMR 1136, Interactions Arbres/Microrganismes (IAM), Labex ARBRE, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Zeller Bernd INRA, Centre de Nancy Lorraine, UR 1138, Biogéochimie des Ecosystèmes Forestiers (BEF), Labex ARBRE, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Plain Caroline INRA, Centre de Nancy Lorraine, UMR 1137, Ecologie et Ecophysiologie Forestières, Labex ARBRE, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Hossann Christian INRA, Centre de Nancy Lorraine, UMR 1137, Ecologie et Ecophysiologie Forestières, Labex ARBRE, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Bréchet Claude INRA, Centre de Nancy Lorraine, UMR 1137, Ecologie et Ecophysiologie Forestières, Labex ARBRE, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Martin Francis INRA, Centre de Nancy Lorraine, UMR 1136, Interactions Arbres/Microrganismes (IAM), Labex ARBRE, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Kohler Annegret INRA, Centre de Nancy Lorraine, UMR 1136, Interactions Arbres/Microrganismes (IAM), Labex ARBRE, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Villerd Jean Université de Lorraine, UMR 1121 « Agronomie & Environnement » Nancy-Colmar, F-54518, Vandoeuvre-les-Nancy, France aut NATIONALLICENCE 700 1- Robin Christophe Université de Lorraine, UMR 1121 « Agronomie & Environnement » Nancy-Colmar, F-54518, Vandoeuvre-les-Nancy, France aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 351-373 0032-079X 395:1-2<351 2015 395 11104