caa a22 4500 606219331 CHVBK 20210128101117.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11104-015-2562-x doi (NATIONALLICENCE)springer-10.1007/s11104-015-2562-x Rhizosphere bacteria and fungi associated with plant growth in soils of three replanted apple orchards [Elektronische Daten] [Ingrid Franke-Whittle, Luisa Manici, Heribert Insam, Blaz Stres] Background and aims: High-throughput 454 pyrosequencing was applied to investigate differences in bacterial and fungal communities between replant and closely situated control non-replant (fallow) soils. Methods: The V1-V3 region of the bacterial 16S rRNA gene and the ITS1 region of fungi from the different soils were sequenced using 454 pyrosequencing (Titanium chemistry), and data were analysed using the MOTHUR pipeline. Results: The bacterial phyla Proteobacteria, Actinobacteria and Acidobacteria dominated in both fallow and replant apple orchard soils, and community composition at both phylum and genus level did not significantly differ according to NP-MANOVA. The fungal phyla Ascomycota, Zygomycota and Basidiomycota were dominant, and communities also did not differ in composition at either phylum or genus level. High positive Pearson correlations with plant growth in a plant growth assay performed with apple rootstocks plantlets were detected for the bacterial genera Gp16 and Solirubrobacter (r: >0.82) and fungal genera Scutellinia, Penicillium, Lecythophora and Paecilomyces (r: >0.65). Strong negative correlations with plant growth were detected for the bacterial genera Chitinophaga and Hyphomicrobium (r: <−0.78) and the fungal genera Acremonium, Fusarium and Cylindrocarpon (r: <−0.81). Conclusions: Study findings are in part consistent with those of previous research, but also highlight associations between apple plants and certain microbial genera. The functional role of these genera in affecting soil health and fertility should be further investigated. Springer International Publishing Switzerland, 2015 Apple replant disease (ARD) nationallicence High-throughput pyrosequencing nationallicence 16S rRNA gene nationallicence ITS nationallicence Franke-Whittle Ingrid Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut Manici Luisa Research Institute for Industrial Crops, Council for Agricultural Research and Economics (CRA), Via di Corticella 133, 40128, Bologna, Italy aut Insam Heribert Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut Stres Blaz Biotechnical Faculty, Department of Animal Science, Group for Microbiology and Microbial Biotechnology, University of Ljubljana, Groblje 3, SI-1230, Domzale, Slovenia aut Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 317-333 0032-079X 395:1-2<317 2015 395 11104 https://doi.org/10.1007/s11104-015-2562-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2562-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Franke-Whittle Ingrid Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Manici Luisa Research Institute for Industrial Crops, Council for Agricultural Research and Economics (CRA), Via di Corticella 133, 40128, Bologna, Italy aut NATIONALLICENCE 700 1- Insam Heribert Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Stres Blaz Biotechnical Faculty, Department of Animal Science, Group for Microbiology and Microbial Biotechnology, University of Ljubljana, Groblje 3, SI-1230, Domzale, Slovenia aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 317-333 0032-079X 395:1-2<317 2015 395 11104