caa a22 4500 606219358 CHVBK 20210128101118.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11104-015-2539-9 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2539-9 The inward-rectifying K+ channel SsAKT1 is a candidate involved in K+ uptake in the halophyte Suaeda salsa under saline condition [Elektronische Daten] [Hui-Rong Duan, Qing Ma, Jin-Lin Zhang, Jing Hu, Ai-Ke Bao, Li Wei, Qian Wang, Sheng Luan, Suo-Min Wang] Background and aims: The Shaker AKT1-like channels are considered to be involved in both high- and low-affinity K+ uptake and correlated with salt tolerance in glycophytes. Suaeda salsa (Suaeda maritima subsp. salsa), as a typical salt-accumulating halophyte, is able to absorb K+ efficiently while growing under saline conditions and taking in a large amount of Na+, thus maintaining the K+ concentration in its cells. In this study, the possible functions of the inward-rectifying K+ channel SsAKT1 in K+ uptake and salt tolerance in the halophyte S. salsa were investigated. Methods: SsAKT1 from S. salsa was isolated by RT-PCR and characterized using yeast complementation; the responses of SsAKT1 to various KCl and NaCl treatments were investigated by real-time quantitative PCR. Results: SsAKT1 consisted of 879 amino acid residues and shared high homology (60-67%) with the identified inward-rectifying K+ channels AKT1 from other plants. The expression of SsAKT1 rescued the K+-uptake-defective phenotype of yeast strain CY162, and also suppressed the salt-sensitive phenotype of yeast strain G19, suggesting SsAKT1 functioned as an inward-rectifying K+ channel. SsAKT1 was predominantly expressed in roots, and was induced significantly by K+ starvation; transcript levels increased further on resupply of K+ (0.1-10mM for 6h) by 62% in 0.1mMK+ and 144-174% in higher K+ concentrations (1-10mM). Interestingly, the expression level of SsAKT1 in roots was also induced significantly by short-term treatment (6h) with NaCl concentrations (25-250mM). Conclusions: These results demonstrate that the inward-rectifying K+ channel SsAKT1 might mediate both high- and low-affinity K+ uptake in S. salsa, but play a greater role in the low-affinity system. Furthermore, SsAKT1 might also be involved in salt tolerance by participating in the maintenance of K+ nutrition in S. salsa under salinity. Springer International Publishing Switzerland, 2015 Suaeda maritima subsp. salsa nationallicence AKT1 nationallicence Yeast complementation nationallicence K+ uptake nationallicence Salt tolerance nationallicence Duan Hui-Rong State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut Ma Qing State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut Zhang Jin-Lin State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut Hu Jing State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut Bao Ai-Ke State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut Wei Li State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut Wang Qian Key Laboratory of Land Surface Process and Climate Change, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730020, Lanzhou, People's Republic of China aut Luan Sheng Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut Wang Suo-Min State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 173-187 0032-079X 395:1-2<173 2015 395 11104 https://doi.org/10.1007/s11104-015-2539-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2539-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Duan Hui-Rong State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ma Qing State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jin-Lin State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Jing State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Bao Ai-Ke State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wei Li State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Qian Key Laboratory of Land Surface Process and Climate Change, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730020, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Luan Sheng Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Wang Suo-Min State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, 730020, Lanzhou, People's Republic of China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 173-187 0032-079X 395:1-2<173 2015 395 11104