caa a22 4500 606219366 CHVBK 20210128101118.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11104-015-2445-1 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2445-1 Induced systemic resistance in tomato ( Solanum lycopersicum ) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling [Elektronische Daten] [Zeraye Mehari, Yigal Elad, Dalia Rav-David, Ellen Graber, Yael Meller Harel] Aims: The goal of this study was to identify the induced resistance pathway mediated by biochar in the tomato - Botrytis cinerea pathosystem. Methods: Tomato wild types and mutants modified in their salicylic acid (SA), ethylene (ET) or jasmonic acid (JA) metabolism were grown in a potting medium amended with biochar produced at 450 ºC from greenhouse wastes, to identify the possible pathway(s) involved in biochar-mediated resistance to B. cinerea. Early cellular response of H2O2 accumulation was biochemically tested, and the transcriptional changes of 12 defense-related genes upon B. cinerea challenge of detached leaflets were analyzed. Results: Biochar amendment resulted in about 50% reduction in B. cinerea disease severity in all tested genotypes with the exception of a JA deficient mutant, def1. Biochar amendment induced priming of early as well as late-acting defense responses particularly in the genes Pti5 (ET-related) and Pi2 (JA-related), which are known to be crucial in resistance against B. cinerea. Stronger and earlier H2O2 accumulation subsequent to B. cinerea inoculation in all genotypes was observed as a result of biochar amendment, with the exception of the def1 mutation. Conclusion: Biochar-mediated IR in the B. cinerea-tomato pathosystem involves the JA pathway. Springer International Publishing Switzerland, 2015 Control agents nationallicence Grey mould nationallicence Induced resistance nationallicence Jasmonic acid nationallicence Plant disease nationallicence Tomato nationallicence Biochar nationallicence AUDPC : Area under disease progress curve nationallicence ET : Ethylene nationallicence IR : Induced resistance nationallicence ISR : Induced systemic resistance nationallicence JA : Jasmonic acid nationallicence PGPR : Plant growth promoting rhizobacteria nationallicence PGPF : Plant growth promoting fungi nationallicence SA : Salicylic acid nationallicence SAR : Systemic acquired resistance nationallicence Mehari Zeraye Department of Plant Pathology and Weed Research, Institute of Plant Protection, The Volcani Center, Agricultural Research Organization, 50250, Bet Dagan, Israel aut Elad Yigal Department of Plant Pathology and Weed Research, Institute of Plant Protection, The Volcani Center, Agricultural Research Organization, 50250, Bet Dagan, Israel aut Rav-David Dalia Department of Plant Pathology and Weed Research, Institute of Plant Protection, The Volcani Center, Agricultural Research Organization, 50250, Bet Dagan, Israel aut Graber Ellen Department of Soil Chemistry, Plant Nutrition and Microbiology, Agricultural Research Organization, The Volcani Center, Institute of Soil, Water and Environmental Sciences, 50250, Bet Dagan, Israel aut Meller Harel Yael Department of Plant Pathology and Weed Research, Institute of Plant Protection, The Volcani Center, Agricultural Research Organization, 50250, Bet Dagan, Israel aut Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 31-44 0032-079X 395:1-2<31 2015 395 11104 https://doi.org/10.1007/s11104-015-2445-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2445-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mehari Zeraye Department of Plant Pathology and Weed Research, Institute of Plant Protection, The Volcani Center, Agricultural Research Organization, 50250, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Elad Yigal Department of Plant Pathology and Weed Research, Institute of Plant Protection, The Volcani Center, Agricultural Research Organization, 50250, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Rav-David Dalia Department of Plant Pathology and Weed Research, Institute of Plant Protection, The Volcani Center, Agricultural Research Organization, 50250, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Graber Ellen Department of Soil Chemistry, Plant Nutrition and Microbiology, Agricultural Research Organization, The Volcani Center, Institute of Soil, Water and Environmental Sciences, 50250, Bet Dagan, Israel aut NATIONALLICENCE 700 1- Meller Harel Yael Department of Plant Pathology and Weed Research, Institute of Plant Protection, The Volcani Center, Agricultural Research Organization, 50250, Bet Dagan, Israel aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 31-44 0032-079X 395:1-2<31 2015 395 11104