caa a22 4500 606219390 CHVBK 20210128101118.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11104-015-2549-7 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2549-7 Plant acquisition and metabolism of the synthetic nitrification inhibitor dicyandiamide and naturally-occurring guanidine from agricultural soils [Elektronische Daten] [Karina Marsden, Matthew Scowen, Paul Hill, Davey Jones, David Chadwick] Background and aims: There is increasing interest and use of nitrification inhibitors (NI) in agroecosystems, yet little is known of their fate in planta. Residues of the organic, N-rich NI, dicyandiamide (DCD), have been found in milk products following commercial application to pasture. We investigated whether plant acquisition and metabolism of DCD were consistent with plant-mediated transmission from soil to agricultural food products. Methods: Uptake rates, translocation to the shoot, degradation of the label within wheat tissue and availability within two soils of DCD and the structurally similar naturally occurring N-rich molecule, guanidine, were measured using 14C labelling. Results: Under sterile conditions, over 2h wheat took up (34 and 14μmolg−1 root DW h−1 at 1mM: DCD and guanidine, respectively), translocated (7-15 and 19-22%) and metabolised (0.4 and 0.9% of uptake) DCD- and guanidine-14C. Both molecules were also acquired from soil by wheat despite concurrent soil sorption and microbial uptake. Conclusions: Both DCD and guanidine can be acquired and metabolised by graminaceous plants. Although probably not a significant route of N acquisition, plant uptake provides a direct route of DCD entry into the food chain. The Author(s), 2015 Bioavailability nationallicence Dicyandiamide (DCD) nationallicence Guanidine nationallicence Mineralization nationallicence Nitrification inhibitor nationallicence Nitrogen cycle nationallicence Marsden Karina School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut Scowen Matthew School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut Hill Paul School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut Jones Davey School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut Chadwick David School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 201-214 0032-079X 395:1-2<201 2015 395 11104 https://doi.org/10.1007/s11104-015-2549-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2549-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marsden Karina School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 700 1- Scowen Matthew School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 700 1- Hill Paul School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 700 1- Jones Davey School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 700 1- Chadwick David School of Environment, Natural Resources and Geography, Bangor University, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 395/1-2(2015-10-01), 201-214 0032-079X 395:1-2<201 2015 395 11104