caa a22 4500 606219978 CHVBK 20210128101121.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11104-015-2536-z doi (NATIONALLICENCE)springer-10.1007/s11104-015-2536-z Waterlogging tolerance in barley is associated with faster aerenchyma formation in adventitious roots [Elektronische Daten] [Xuechen Zhang, Sergey Shabala, Anthony Koutoulis, Lana Shabala, Peter Johnson, Dane Hayes, David Nichols, Meixue Zhou] Background and aims: Plant adaptation to waterlogged conditions requires a set of morphological and physiological/biochemical changes. The formation of aerenchyma is one of the most crucial adaptive traits for waterlogging tolerance. Enzymatic scavenging may also potentially contribute to waterlogging tolerance by providing detoxification of reactive oxygen species (ROS). Methods: Changes of root porosity (as an indicator of aerenchyma formation) and activities in leaves of four major antioxidant enzymes, γ-amino butyric acid (GABA) and lactic acid contents in roots were evaluated in six barley genotypes contrasting in waterlogging tolerance. Results: Soil waterlogging caused significant increases in adventitious root porosity in all genotypes. Waterlogging-tolerant genotypes showed not only significantly higher adventitious root porosity than sensitive genotypes but also much faster development of aerenchyma. The greatest difference in adventitious root porosity among genotypes was observed after 7days of waterlogging treatment. At the same time, antioxidant enzyme activities in leaves, GABA and lactic acid contents in roots did not correlate with waterlogging tolerance. Conclusions: A faster formation of aerenchyma in adventitious roots is one of the key factors for waterlogging tolerance in barley. This protocol is recommended to be applied in future studies to identify molecular markers linked to this trait using appropriate mapping populations. Springer International Publishing Switzerland, 2015 Barley nationallicence Waterlogging tolerance nationallicence Root aerenchyma nationallicence Root porosity nationallicence Antioxidant enzymes nationallicence Reactive oxygen species nationallicence Zhang Xuechen School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut Shabala Sergey School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut Koutoulis Anthony School of Biological Sciences, University of Tasmania, Private Bag 55, 7001, Hobart, TAS, Australia aut Shabala Lana School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut Johnson Peter School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut Hayes Dane Department of Primary Industries, Water and Environment, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut Nichols David Central Science Laboratory, University of Tasmania, Private Bag 74, 7001, Hobart, TAS, Australia aut Zhou Meixue School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut Plant and Soil Springer International Publishing 394/1-2(2015-09-01), 355-372 0032-079X 394:1-2<355 2015 394 11104 https://doi.org/10.1007/s11104-015-2536-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2536-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Xuechen School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut NATIONALLICENCE 700 1- Shabala Sergey School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut NATIONALLICENCE 700 1- Koutoulis Anthony School of Biological Sciences, University of Tasmania, Private Bag 55, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Shabala Lana School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut NATIONALLICENCE 700 1- Johnson Peter School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut NATIONALLICENCE 700 1- Hayes Dane Department of Primary Industries, Water and Environment, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut NATIONALLICENCE 700 1- Nichols David Central Science Laboratory, University of Tasmania, Private Bag 74, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Zhou Meixue School of Land and Food, University of Tasmania, P.O. Box 46, 7249, Kings Meadows, TAS, Australia aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 394/1-2(2015-09-01), 355-372 0032-079X 394:1-2<355 2015 394 11104