caa a22 4500 606219994 CHVBK 20210128101121.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11104-015-2530-5 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2530-5 Organic acids metabolism in roots of grapevine rootstocks under severe iron deficiency [Elektronische Daten] [José Covarrubias, Adamo Rombolà] Background and aims: In many important viticultural areas of the Mediterranean basin, plants often face prolonged periods of scarce iron (Fe) availability in the soil. The objective of the present work was to perform a comparative analysis of physiological and biochemical responses of Vitis genotypes to severe Fe deficiency. Methods: Three grapevine rootstocks differing in susceptibility to Fe chlorosis were grown with and without Fe in the nutrient solution. Results: Rootstock 101-14, susceptible to Fe chlorosis, responded to severe Fe deficiency by reducing the root activity of phosphoenolpyruvate carboxylase (PEPC) and malate dehydrogenase (MDH), however, it accumulated high levels of citric acid. By contrast, rootstock 110 Richter, tolerant to Fe chlorosis, maintained an active metabolism of organic acids, but citric acid accumulation was lower than in 101-14. Similarly to 101-14, rootstock SO4 showed a strong decrease in PEPC and MDH activities. Nevertheless it maintained moderate citric acid levels in the roots, mimicking the response by 110 Richter. Conclusions: Root PEPC and MDH activities can be used as tools for screening Fe chlorosis tolerance. Conversely, organic acids accumulation in roots may not be a reliable indicator of Fe chlorosis tolerance, particularly under conditions of severe Fe deficiency, because of their probable exudation by roots. Our results show that drawing sound conclusions from screening programs involving Fe deficiency tolerance requires short as well as long-term assessment of responses to Fe deprivation. Springer International Publishing Switzerland, 2015 Iron chlorosis nationallicence Phosphoenolpyruvate carboxylase nationallicence Organic acids nationallicence Enzyme activity nationallicence Grapevine genotypes nationallicence BSA : Bovine serum albumin nationallicence CoA : Coenzyme A nationallicence CS : Citrate synthase nationallicence DW : Dry weight nationallicence EDTA : Ethylenediaminetetraacetic acid nationallicence FW : Fresh weight nationallicence MDH : Malate dehydrogenase nationallicence NADP+-IDH : Isocitrate dehydrogenase nationallicence PEPC : Phosphoenolpyruvate carboxylase nationallicence TCA : Tricarboxylic acid nationallicence Covarrubias José Facultad de Ciencias Agronómicas, Universidad de Chile, Av. Santa Rosa, 11315, Santiago, Chile aut Rombolà Adamo Department of Agricultural Sciences, University of Bologna, Viale G. Fanin 44, 40127, Bologna, Italy aut Plant and Soil Springer International Publishing 394/1-2(2015-09-01), 165-175 0032-079X 394:1-2<165 2015 394 11104 https://doi.org/10.1007/s11104-015-2530-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2530-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Covarrubias José Facultad de Ciencias Agronómicas, Universidad de Chile, Av. Santa Rosa, 11315, Santiago, Chile aut NATIONALLICENCE 700 1- Rombolà Adamo Department of Agricultural Sciences, University of Bologna, Viale G. Fanin 44, 40127, Bologna, Italy aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 394/1-2(2015-09-01), 165-175 0032-079X 394:1-2<165 2015 394 11104