caa a22 4500 606220119 CHVBK 20210128101122.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11104-015-2506-5 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2506-5 Diversity and functional characterization of bacterial endophytes dwelling in various rice ( Oryza sativa L.) tissues, and their seed-borne dissemination into rhizosphere under gnotobiotic P-stress [Elektronische Daten] [Asif Hameed, Meng-Wei Yeh, Yu-Ting Hsieh, Wei-Ching Chung, Chaur-Tsuen Lo, Li-Sen Young] Aim: Endophytic bacterial diversity in four rice cultivars grown in two soil-types, their plant-probiotic features and rhizospheric deployment under P-stress were investigated. Methods: Oryza sativa cvs. TCN1, TCS10, TK8, and TN71 were cultivated in greenhouse using non-sterile acidic and near-neutral paddy soils for 60days. Root, stem and leaf tissues were screened for culturable bacterial endophytes using nutrient agar. Endophytes were identified and profiled for plant-probiotic features. The richness, Shannon-Weiner diversity, evenness and Venn's distribution in terms of endophytic strains were evaluated. Seed-borne endophytes were characterized through DGGE. The deployment of endophytes into the rhizosphere in TCN1 and TK8 under gnotobiotic P-stress was assessed. Results: A total of 52 distinct endophytic bacterial strains affiliated to 5 classes and 20 discrete genera exhibiting differential plant-probiotic features were isolated from various tissues of four different rice cultivars. The diversity and distribution of endophytes fluctuated with soil-type, tissue-type and rice genotype. Gnotobiotic insoluble P treatment revealed significantly enhanced deployment of P-solubilizing rhizobacteria in TCN1 as compared to soluble P and P-lacking control. Conclusions: Rice endophytic bacteria are diverse, and their distribution within the plant and deployment as rhizobacteria were found to be influenced by host genotype, edaphic factors and nutrient stress. Springer International Publishing Switzerland, 2015 Rice endophytes nationallicence Shannon-Weiner index nationallicence Siderophore nationallicence Biocontrol nationallicence Diazotroph nationallicence P-solubilizing bacteria nationallicence Nutrient stress nationallicence Hameed Asif Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, No.250, Kuo Kuang Road, 40227, Taichung, Taiwan aut Yeh Meng-Wei Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, No.64, Wunhua Rd., Huwei Township, 632, Yunlin, Taiwan aut Hsieh Yu-Ting Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, No.250, Kuo Kuang Road, 40227, Taichung, Taiwan aut Chung Wei-Ching Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, No.64, Wunhua Rd., Huwei Township, 632, Yunlin, Taiwan aut Lo Chaur-Tsuen Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, No.64, Wunhua Rd., Huwei Township, 632, Yunlin, Taiwan aut Young Li-Sen Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, No.64, Wunhua Rd., Huwei Township, 632, Yunlin, Taiwan aut Plant and Soil Springer International Publishing 394/1-2(2015-09-01), 177-197 0032-079X 394:1-2<177 2015 394 11104 https://doi.org/10.1007/s11104-015-2506-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2506-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hameed Asif Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, No.250, Kuo Kuang Road, 40227, Taichung, Taiwan aut NATIONALLICENCE 700 1- Yeh Meng-Wei Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, No.64, Wunhua Rd., Huwei Township, 632, Yunlin, Taiwan aut NATIONALLICENCE 700 1- Hsieh Yu-Ting Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, No.250, Kuo Kuang Road, 40227, Taichung, Taiwan aut NATIONALLICENCE 700 1- Chung Wei-Ching Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, No.64, Wunhua Rd., Huwei Township, 632, Yunlin, Taiwan aut NATIONALLICENCE 700 1- Lo Chaur-Tsuen Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, No.64, Wunhua Rd., Huwei Township, 632, Yunlin, Taiwan aut NATIONALLICENCE 700 1- Young Li-Sen Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, No.64, Wunhua Rd., Huwei Township, 632, Yunlin, Taiwan aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 394/1-2(2015-09-01), 177-197 0032-079X 394:1-2<177 2015 394 11104