caa a22 4500 606220135 CHVBK 20210128101122.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11104-015-2497-2 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2497-2 Biofumigation using a wild Brassica oleracea accession with high glucosinolate content affects beneficial soil invertebrates [Elektronische Daten] [Diana Zuluaga, A. van Ommen Kloeke, Ruud Verkerk, Wilfred Röling, Jacintha Ellers, Dick Roelofs, Mark Aarts] Aims: This study explores the biofumigation effects of glucosinolate (GSL) containing Brassica oleracea plant material on beneficial, non-target soil organisms, and aims to relate those effects to differences in GSL profiles. Methods: Leaf material of purple sprouting broccoli ‘Santee', Savoy cabbage ‘Wintessa', and the wild B. oleracea accession Winspit was analysed for GSL production and used for biofumigation experiments on the beneficial soil invertebrates, Folsomia candida (springtail) and Eisenia andrei (earthworm) and the soil bacterial community. Results: When mixed into soil, the Winspit plant material exerted the highest toxic effects on beneficial soil invertebrates by reducing survival and reproduction. Total GSL levels varied substantially between genotypes, in particular the aliphatic GSL (AGSL) sinigrin and gluconapin being highly abundant or exclusively present in Winspit. Differences between the genotypes regarding biofumigation effects on the soil microbial community were only observed on a temporal basis with the largest difference in bacterial community structure after 1week. Conclusions: The high total GSL content in biofumigated soil could explain the toxicity of Winspit for soil invertebrates. These effects are likely to be the results of high AGSL levels in Winspit. The use of wild B. oleracea crops, such as Winspit, for biofumigation practices would need a proper assessment of the overall impact on soil biota before being applied on a wide scale. The Author(s), 2015 Brassicaceae nationallicence Eisenia andrei nationallicence Folsomia candida nationallicence Gluconapin nationallicence Sinigrin nationallicence Winspit nationallicence Zuluaga Diana Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut van Ommen Kloeke A. Department of Ecological Science, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands aut Verkerk Ruud Food Quality and Design, Wageningen University, P.O. Box 17, 6700AA, Wageningen, The Netherlands aut Röling Wilfred Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands aut Ellers Jacintha Department of Ecological Science, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands aut Roelofs Dick Department of Ecological Science, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands aut Aarts Mark Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut Plant and Soil Springer International Publishing 394/1-2(2015-09-01), 155-163 0032-079X 394:1-2<155 2015 394 11104 https://doi.org/10.1007/s11104-015-2497-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2497-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zuluaga Diana Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van Ommen Kloeke A. Department of Ecological Science, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Verkerk Ruud Food Quality and Design, Wageningen University, P.O. Box 17, 6700AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Röling Wilfred Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Ellers Jacintha Department of Ecological Science, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Roelofs Dick Department of Ecological Science, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Aarts Mark Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 394/1-2(2015-09-01), 155-163 0032-079X 394:1-2<155 2015 394 11104