caa a22 4500 606220240 CHVBK 20210128101122.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11104-015-2493-6 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2493-6 Sampling root-respired CO2 in-situ for 13C measurement [Elektronische Daten] [Helen Snell, David Robinson, Andrew Midwood] Background and aims: Root-respired δ13CO2 can be useful for exploring plant carbon allocation and root respiratory fractionation as well as for partitioning soil-surface CO2 emissions into plant root and soil organic matter (SOM) sources, a necessary measure for calculating the contribution of heterotrophic respiration of soil carbon to net ecosystem exchange. Root CO2 is usually sampled from excised roots, however, excision alters respiration rate and isolates the root sample from aboveground plant processes. Methods: To improve the integrity of root efflux δ13CO2 measurements, we designed a chamber for sampling root-respired CO2 in situ from minimally disturbed tree roots. We compared root δ13CO2 values from excised and attached roots in the field and we pruned mature Scots pine trees to induce a measureable change in root δ13CO2. Results: Excised root samples containing root wounds gave more 13C-depleted measurements of root-respired δ13CO2 than intact roots by 1.8 ‰. Using chambers to sample CO2 from attached roots, we measured a diurnal change in root-respired δ13CO2 of 3-4 ‰, triggered by pruning foliage from the trees. Conclusions: This chamber system permits high-frequency sampling of live root-respired δ13CO2 that enables greater insight into plant respiratory processes and more accurate partitioning of soil-surface CO2 emissions. Springer International Publishing Switzerland, 2015 Root respiration nationallicence Carbon nationallicence Stable isotopes nationallicence Soil respiration nationallicence Sampling method nationallicence Carbon dioxide nationallicence Tree roots nationallicence Chamber nationallicence δ13CO2 nationallicence Snell Helen Plant & Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, St Machar Drive, AB24 3UL, Aberdeen, UK aut Robinson David Plant & Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, St Machar Drive, AB24 3UL, Aberdeen, UK aut Midwood Andrew Environmental and Biochemical Sciences, James Hutton Institute, Craigiebuckler, AB15 8QH, Aberdeen, UK aut Plant and Soil Springer International Publishing 393/1-2(2015-08-01), 259-271 0032-079X 393:1-2<259 2015 393 11104 https://doi.org/10.1007/s11104-015-2493-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2493-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Snell Helen Plant & Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, St Machar Drive, AB24 3UL, Aberdeen, UK aut NATIONALLICENCE 700 1- Robinson David Plant & Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, St Machar Drive, AB24 3UL, Aberdeen, UK aut NATIONALLICENCE 700 1- Midwood Andrew Environmental and Biochemical Sciences, James Hutton Institute, Craigiebuckler, AB15 8QH, Aberdeen, UK aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 393/1-2(2015-08-01), 259-271 0032-079X 393:1-2<259 2015 393 11104