caa a22 4500 606220399 CHVBK 20210128101123.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11104-015-2475-8 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2475-8 H2S acting as a downstream signaling molecule of SA regulates Cd tolerance in Arabidopsis [Elektronische Daten] [Zengjie Qiao, Tao Jing, Zhiqiang Liu, Liping Zhang, Zhuping Jin, Danmei Liu, Yanxi Pei] Background and aims: In plants, salicylic acid (SA) acting as one of the signaling molecules can regulate and respond to cadmium (Cd) stress. It is well known that hydrogen sulfide (H2S) could alleviate toxicity of Cd stress but the crosstalk between SA and H2S in regulating Cd stress still remains unclear. Methods: We studied on it through the physiological and biochemical method together with the microstructure synthesis. Results: Our results indicated that the plants exhibited enhanced tolerance to Cd when pretreated with SA, which included weakening oxidative damage and intensifying photosynthesis as well as boosting L-cysteine desulfhydrase (LCD) activity and raising the content of endogenous H2S. In parallel, the plants pretreated with NaHS, a donor of H2S, showed a stronger ability to tolerate Cd stress, SA- pretreated plants presented normal growth and meanwhile the content of chlorophyll and the microstructure of roots were significantly different compared to those treated with Cd only. By contrast, with the same treatments, the positive function and effect of SA on the LCD-knockout mutants, lcd was not observed. Pharmacological experiments further testified these results. Conclusions: All of the results suggest that H2S may be a downstream signal molecule in SA-induced Cd tolerance of Arabidopsis. Springer International Publishing Switzerland, 2015 Hydrogen sulfide nationallicence Salicylic acid nationallicence Cadmium nationallicence Arabidopsis nationallicence Root characteristics nationallicence AOA : Aminooxyacetic acid nationallicence Cd : Cadmium nationallicence LCD : L-cysteine desulfhydrase nationallicence HA : Hydroxylamine nationallicence H2S : Hydrogen sulfide nationallicence HT : Hypotaurine nationallicence MDA : Malondialdehyde nationallicence ROS : Reactive oxygen species nationallicence SA : Salicylic acid nationallicence PP : Potassium pyruvate nationallicence Qiao Zengjie School of Life Science, Shanxi University, 030006, Taiyuan, China aut Jing Tao Department of chemistry and biochemistry, Ohio University, 45701, Athens, OH, USA aut Liu Zhiqiang School of Life Science, Shanxi University, 030006, Taiyuan, China aut Zhang Liping School of Life Science, Shanxi University, 030006, Taiyuan, China aut Jin Zhuping School of Life Science, Shanxi University, 030006, Taiyuan, China aut Liu Danmei School of Life Science, Shanxi University, 030006, Taiyuan, China aut Pei Yanxi School of Life Science, Shanxi University, 030006, Taiyuan, China aut Plant and Soil Springer International Publishing 393/1-2(2015-08-01), 137-146 0032-079X 393:1-2<137 2015 393 11104 https://doi.org/10.1007/s11104-015-2475-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2475-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qiao Zengjie School of Life Science, Shanxi University, 030006, Taiyuan, China aut NATIONALLICENCE 700 1- Jing Tao Department of chemistry and biochemistry, Ohio University, 45701, Athens, OH, USA aut NATIONALLICENCE 700 1- Liu Zhiqiang School of Life Science, Shanxi University, 030006, Taiyuan, China aut NATIONALLICENCE 700 1- Zhang Liping School of Life Science, Shanxi University, 030006, Taiyuan, China aut NATIONALLICENCE 700 1- Jin Zhuping School of Life Science, Shanxi University, 030006, Taiyuan, China aut NATIONALLICENCE 700 1- Liu Danmei School of Life Science, Shanxi University, 030006, Taiyuan, China aut NATIONALLICENCE 700 1- Pei Yanxi School of Life Science, Shanxi University, 030006, Taiyuan, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 393/1-2(2015-08-01), 137-146 0032-079X 393:1-2<137 2015 393 11104