caa a22 4500 606220437 CHVBK 20210128101123.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11104-015-2481-x doi (NATIONALLICENCE)springer-10.1007/s11104-015-2481-x Analysing uncertainties in the calculation of fluxes using whole-plant chambers: random and systematic errors [Elektronische Daten] [Oscar Pérez-Priego, Ana López-Ballesteros, Enrique Sánchez-Cañete, Penélope Serrano-Ortiz, Lars Kutzbach, Francisco Domingo, Werner Eugster, Andrew Kowalski] Aims: Gas exchange measurements on individual plants depend largely on chamber systems, and uncertainties and corrections in current flux calculation procedures require further assessment. Methods: We present a practical study with novel methods for analyses of flux uncertainties in an original chamber design excluding soil fluxes and allowing simultaneous measurements of whole-plant photosynthesis and transpiration. Results: Results indicate that random errors caused by IRGA noise and the lack of criteria to optimize the time window (TW) of chamber enclosure lead to significant flux uncertainties (12%). Although enclosure should be rapid to minimize plant disturbances, longer TWs (3min) increase confidence in flux estimates. Indeterminate stabilization periods in existing calculation protocols cause significant systematic errors. Stabilization times were identified via the change-point detection method, and flux uncertainties were reduced. Photosynthesis was overestimated by up to 28% when not correcting the evolving CO2 molar fraction for water vapour dilution. Leakage can compromise flux estimates, but was negligible (ca. 2%) here due to the large chamber-headspace and relatively small values of both collar contact length and closure time. Conclusions: A bootstrapping, resampling-based flux calculation method is presented and recommended to better assess random errors and improve flux precision. We present practical recommendations for the use of whole-plant chambers. Springer International Publishing Switzerland, 2015 Canopy chamber nationallicence Closed chamber nationallicence Flux calculation methods nationallicence Flux uncertainties nationallicence Shrublands nationallicence Pérez-Priego Oscar Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut López-Ballesteros Ana Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut Sánchez-Cañete Enrique Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut Serrano-Ortiz Penélope Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut Kutzbach Lars Institut für Bodenkunde, University of Hamburg, Allende-Platz 2, 20146, Hamburg, Germany aut Domingo Francisco Departamento de Desertificación y Geoecología, Estación Experimental de Zonas Áridas, Consejo Superior de Investigaciones Científicas, Carretera de Sacramento s/n, La Cañada de San Urbano, 04120, Almería, Spain aut Eugster Werner Institute of Agricultural Sciences, ETH Zurich, Universitätstrasse 2, 8092, Zürich, Switzerland aut Kowalski Andrew Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut Plant and Soil Springer International Publishing 393/1-2(2015-08-01), 229-244 0032-079X 393:1-2<229 2015 393 11104 https://doi.org/10.1007/s11104-015-2481-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2481-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pérez-Priego Oscar Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut NATIONALLICENCE 700 1- López-Ballesteros Ana Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Sánchez-Cañete Enrique Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Serrano-Ortiz Penélope Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Kutzbach Lars Institut für Bodenkunde, University of Hamburg, Allende-Platz 2, 20146, Hamburg, Germany aut NATIONALLICENCE 700 1- Domingo Francisco Departamento de Desertificación y Geoecología, Estación Experimental de Zonas Áridas, Consejo Superior de Investigaciones Científicas, Carretera de Sacramento s/n, La Cañada de San Urbano, 04120, Almería, Spain aut NATIONALLICENCE 700 1- Eugster Werner Institute of Agricultural Sciences, ETH Zurich, Universitätstrasse 2, 8092, Zürich, Switzerland aut NATIONALLICENCE 700 1- Kowalski Andrew Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Avenida Fuentenueva, S/N, 18071, Granada, Spain aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 393/1-2(2015-08-01), 229-244 0032-079X 393:1-2<229 2015 393 11104