caa a22 4500 606221190 CHVBK 20210128101127.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00380-014-0497-4 doi (NATIONALLICENCE)springer-10.1007/s00380-014-0497-4 The expression of p66shc in peripheral blood monocytes is increased in patients with coronary heart disease and correlated with endothelium-dependent vasodilatation [Elektronische Daten] [Qin Miao, Qiong Wang, Lini Dong, Yanjiao Wang, Yi Tan, Xiangyu Zhang] The objective of this study is to detect the p66shc mRNA and protein expression of the peripheral blood monocytes (PBMs) in coronary heart disease patients (CHD) and controls, to evaluate the correlation between the expression of p66shc mRNA in the PBMs and endothelium-dependent vasodilatation. This study included 78 coronary angiography-documented CHD patients (CHD group) and 38 non-CHD controls (control group). The p66shc mRNA and protein levels were determined by quantitative real-time PCR and western blotting. The flow-mediated dilatation (FMD, endothelium-dependent), nitroglycerine-induced dilatation (NID, endothelium-independent) and carotid intimal medial thickness (CIMT) were detected using high-resolution ultrasound. The p66shc mRNA and the protein expression levels in the PBMs were significantly higher in the CHD group compared with the control group (p=0.007 and 0.001). The FMD (p<0.001) and NID (p=0.013) were significantly lower and the CIMT (p=0.007) was significantly thicker in the CHD patients than in the controls. In the univariate analysis, the expression of the p66shc mRNA in the PBMs was significantly positively correlated with the serum LDL-C and homocysteine levels and the CIMT and was inversely correlated with the FMD and the NID (all p<0.001). In the multiple linear regression analysis, the FMD (p<0.001), LDL-C (p=0.002) and homocysteine levels (p=0.002) remained independently correlated with the p66shc mRNA expression. These findings highlight a pivotal role for the expression of p66shc in CHD and endothelial dysfunction, which might represent a molecular target to prevent endothelial dysfunction-related disease. Springer Japan, 2014 p66shc nationallicence Endothelium-dependent vasodilatation nationallicence Coronary heart disease nationallicence Peripheral blood monocytes nationallicence Homocysteine nationallicence Miao Qin Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Wang Qiong Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Dong Lini Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Wang Yanjiao Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Tan Yi Department of Ultrasound, Second Xiangya Hospital of Central South University, 410011, Changsha, Hunan, People's Republic of China aut Zhang Xiangyu Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Heart and Vessels Springer Japan 30/4(2015-07-01), 451-457 0910-8327 30:4<451 2015 30 380 https://doi.org/10.1007/s00380-014-0497-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00380-014-0497-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miao Qin Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Qiong Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Dong Lini Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yanjiao Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Tan Yi Department of Ultrasound, Second Xiangya Hospital of Central South University, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xiangyu Department of Geriatrics, Second Xiangya Hospital of Central South University, No. 139, Middle Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 773 0- Heart and Vessels Springer Japan 30/4(2015-07-01), 451-457 0910-8327 30:4<451 2015 30 380