caa a22 4500 606221506 CHVBK 20210128101129.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00380-014-0499-2 doi (NATIONALLICENCE)springer-10.1007/s00380-014-0499-2 GABAB receptors are expressed in human aortic smooth muscle cells and regulate the intracellular Ca2+ concentration [Elektronische Daten] [Xu-Ping Wang, Zhen-Ying Cheng, Katrina Schmid] The aim of this study was to investigate the expression of GABAB receptors, a subclass of receptors to the inhibitory neurotransmitter gamma-aminobutyric acid (GABAB), in human aortic smooth muscle cells (HASMCs), and to explore if altering receptor activation modified intracellular Ca2+ concentration ([Ca2+]i) of HASMCs. Real-time PCR, western blots and immunofluorescence were used to determine the expression of GABABR1 and GABABR2 in cultured HASMCs. Immunohistochemistry was used to localize the two subunits in human left anterior descending artery (LAD). The effects of the GABAB receptor agonist baclofen on [Ca2+]i in cultured HASMCs were demonstrated using fluo-3. Both GABABR1 and GABABR2 mRNA and protein were identified in cultured HASMCs and antibody staining was also localized to smooth muscle cells of human LAD. 100μM baclofen caused a transient increase of [Ca2+]i in cultured HASMCs regardless of whether Ca2+ was added to the medium, and the effects were inhibited by pre-treatment with CGP46381 (selective GABAB receptor antagonist), pertussis toxin (a Gi/o protein inhibitor), and U73122 (a phospholipase C blocker). GABAB receptors are expressed in HASMCs and regulate the [Ca2+]i via a Gi/o-coupled receptor pathway and a phospholipase C activation pathway. Springer Japan, 2014 Human aortic smooth muscle cells nationallicence GABAB receptors nationallicence Calcium nationallicence GABA : γ-Aminobutyric acid nationallicence GABABR1 : γ-Aminobutyric acid type B receptor subunit one nationallicence GABABR2 : γ-Aminobutyric acid type B receptor subunit two nationallicence HASMCs : Human aortic smooth muscle cells nationallicence VSMCs : Vascular smooth muscle cells nationallicence PCR : Polymerase chain reaction nationallicence mRNA : Messenger RNA nationallicence G proteins : Guanine nucleotide-binding proteins nationallicence [Ca2+]i : Intracellular Ca2+ concentration nationallicence SMCM : Smooth muscle cell medium nationallicence LAD : Left anterior descending artery nationallicence Wang Xu-Ping The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Qilu Hospital, Shandong University, Jinan, Shandong, China aut Cheng Zhen-Ying Department of Ophthalmology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, 250012, Jinan, Shandong, China aut Schmid Katrina School of Optometry and Vision Science, Faculty of Health and Vision Improvement Domain, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia aut Heart and Vessels Springer Japan 30/2(2015-03-01), 249-257 0910-8327 30:2<249 2015 30 380 https://doi.org/10.1007/s00380-014-0499-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00380-014-0499-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Xu-Ping The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Qilu Hospital, Shandong University, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Cheng Zhen-Ying Department of Ophthalmology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, 250012, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Schmid Katrina School of Optometry and Vision Science, Faculty of Health and Vision Improvement Domain, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia aut NATIONALLICENCE 773 0- Heart and Vessels Springer Japan 30/2(2015-03-01), 249-257 0910-8327 30:2<249 2015 30 380