caa a22 4500 606227253 CHVBK 20210128101157.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10641-015-0402-8 doi (NATIONALLICENCE)springer-10.1007/s10641-015-0402-8 The endangered species Brycon orbignyanus [Elektronische Daten] genetic analysis and definition of priority areas for conservation [Fernando Ashikaga, Mario Orsi, Claudio Oliveira, José Senhorini, Fausto Foresti] Currently, biodiversity is threatened by several factors often associated with human population growth and the extension of areas occupied by human activity. In particular, freshwater fish fauna is affected by overfishing, deforestation, water pollution, introduction of exotic species and habitat fragmentation promoted by hydroelectric dams, among other environmental impact factors. Several action plans to preserve ichthyofauna biodiversity have been adopted; however, these plans frequently cover only a small number of species, and decisions are often made without strong scientific support. This study aimed to evaluate the genetic aspects of wild groups of Brycon orbignyanus, an endangered fish species, using microsatellites and D-loop regions to identify the genetic structure of the samples and to establish priority areas for conservation based on the genetic patterns of this species. The results indicated that the samples showed levels of genetic variability compatible with others studies with Neotropical fishes. However, the results obtained in the analysis of molecular variance (AMOVA) for microsatellites (F ST = 0.258) and D-loop (F ST = 0.234) and the interpopulation fixation index revealed that B. orbignyanus was structured in different subpopulations in the La Plata River basin; the areas with better environmental conditions also showed subgroups with higher rates of genetic variability. Future conservation actions addressing these sites should consider two different management units: the complex formed by the Ivinhema River, Upper Paraná, Camargo Port and Ilha Grande groups; and the complex formed by the Verde River and Sucuriu River groups. Springer Science+Business Media Dordrecht, 2015 Genetic conservation nationallicence Genetic structure nationallicence Genetic variability nationallicence Freshwater fish nationallicence Migratory species nationallicence Environmental management nationallicence Ashikaga Fernando Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, s/n, 18618-970, Botucatu, SP, Brazil aut Orsi Mario Departamento de Biologia Animal e Vegetal, Universidade Estadual de Londrina, Londrina, PR, Brazil aut Oliveira Claudio Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, s/n, 18618-970, Botucatu, SP, Brazil aut Senhorini José Centro Nacional de Pesquisa e Conservação de Peixes Continentais, CEPTA/ICMBio, Pirassununga, SP, Brazil aut Foresti Fausto Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, s/n, 18618-970, Botucatu, SP, Brazil aut Environmental Biology of Fishes Springer Netherlands 98/7(2015-07-01), 1845-1855 0378-1909 98:7<1845 2015 98 10641 https://doi.org/10.1007/s10641-015-0402-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10641-015-0402-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ashikaga Fernando Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, s/n, 18618-970, Botucatu, SP, Brazil aut NATIONALLICENCE 700 1- Orsi Mario Departamento de Biologia Animal e Vegetal, Universidade Estadual de Londrina, Londrina, PR, Brazil aut NATIONALLICENCE 700 1- Oliveira Claudio Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, s/n, 18618-970, Botucatu, SP, Brazil aut NATIONALLICENCE 700 1- Senhorini José Centro Nacional de Pesquisa e Conservação de Peixes Continentais, CEPTA/ICMBio, Pirassununga, SP, Brazil aut NATIONALLICENCE 700 1- Foresti Fausto Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, s/n, 18618-970, Botucatu, SP, Brazil aut NATIONALLICENCE 773 0- Environmental Biology of Fishes Springer Netherlands 98/7(2015-07-01), 1845-1855 0378-1909 98:7<1845 2015 98 10641