caa a22 4500 60622792X CHVBK 20210128101201.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10641-015-0405-5 doi (NATIONALLICENCE)springer-10.1007/s10641-015-0405-5 Environmental DNA as an effective tool for detection of imperiled fishes [Elektronische Daten] [Alexis Janosik, Carol Johnston] Detection of aquatic species is imperfect, especially if the species is rare and exhibits spatial and temporal variability. Many fish species require a number of sampling trips before detection is positive. And yet, information on species persistence is critical for effective conservation efforts. New forensic genetic techniques, such as environmental DNA (eDNA), have been developed and successfully used to validate the presence of exotic aquatic species in new areas. We compared detection of a federally listed, threatened, migratory fish species the Slackwater darter (Etheostoma boschungi); using eDNA to concomitantly collected field collections. Detection probabilities for this species are variable, but consistently low in recent collections. Our results indicated that detection using eDNA was vastly more effective than traditional sampling at confirming the presence of the Slackwater darter. Positive detection at non-breeding sites was half of the detection rate at breeding sites, most likely to the greater area available in non-breeding streams. These data suggest that eDNA is an effective tool for quickly evaluating a relatively large number of sites for the presence of rare aquatic species. Springer Science+Business Media Dordrecht, 2015 Environmental DNA nationallicence Biodiversity inventories nationallicence Percidae nationallicence Slackwater darter nationallicence Janosik Alexis Department of Biology, University of West Florida, 32503, Pensacola, FL, USA aut Johnston Carol Fish Biodiversity Lab, Department of Fisheries, Auburn University, 36849, Auburn, AL, USA aut Environmental Biology of Fishes Springer Netherlands 98/8(2015-08-01), 1889-1893 0378-1909 98:8<1889 2015 98 10641 https://doi.org/10.1007/s10641-015-0405-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10641-015-0405-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Janosik Alexis Department of Biology, University of West Florida, 32503, Pensacola, FL, USA aut NATIONALLICENCE 700 1- Johnston Carol Fish Biodiversity Lab, Department of Fisheries, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 773 0- Environmental Biology of Fishes Springer Netherlands 98/8(2015-08-01), 1889-1893 0378-1909 98:8<1889 2015 98 10641