caa a22 4500 606228403 CHVBK 20210128101203.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10641-015-0443-z doi (NATIONALLICENCE)springer-10.1007/s10641-015-0443-z High resolution profiles of elements in Atlantic tarpon ( Megalops atlanticus ) scales obtained via cross-sectioning and laser ablation ICP-MS: a literature survey and novel approach for scale analyses [Elektronische Daten] [Matthew Seeley, Nathaniel Miller, Benjamin Walther] Fish scales may be used as nonlethal alternative structures to otoliths for establishing chemical time-series that trace migration and habitat use for euryhaline species such as Atlantic tarpon (Megalops atlanticus). We survey four decades of fish scale studies employing elemental analyses to show the wide diversity of aims, approaches and instrumentation. Analyses are mainly solution-based measurements of whole or subsampled scales or in situ solid-sampling techniques of specific growth regions. Although spot-based transects across scales have been employed for a handful of species, continuous elemental transects across scale growth increments are rare. Laser ablation based sampling methods that assay scale exteriors can be difficult to implement for species with natural topographic variations exceeding the focal range of the laser. These limitations may be overcome by performing laser ablation of scales in cross-section when scale thickness in the well-calcified external layer provides enough surface area for a laser transect. For this study, scales of Atlantic tarpon were embedded in epoxy and longitudinally cross-sectioned to expose the scale growth record of the external layer. Laser ablation ICP-MS was used to quantify continuous profiles of element:Ca ratios across scale growth increments. The analytes surveyed (Mg, P, Ca, Mn, Sr, and Ba) were consistently above the limits of detection (LODs) across each scale with the exception of Pb for one scale. Obtaining high resolution elemental life history profiles from scales will be essential for future efforts to use this structure as a non-lethal substitute for otoliths in Atlantic tarpon and other migratory fishes that possess scales with sufficiently thick calcified external layers to permit a cross-sectional approach. Springer Science+Business Media Dordrecht, 2015 Atlantic tarpon nationallicence Scales nationallicence Otoliths nationallicence ICP-MS nationallicence Laser ablation nationallicence Seeley Matthew Marine Science Institute, University of Texas at Austin, 750 Channel View Drive, 78373, Port Aransas, TX, USA aut Miller Nathaniel Jackson School of Geosciences, University of Texas at Austin, 23rd St, 78759, Austin, TX, USA aut Walther Benjamin Marine Science Institute, University of Texas at Austin, 750 Channel View Drive, 78373, Port Aransas, TX, USA aut Environmental Biology of Fishes Springer Netherlands 98/11(2015-11-01), 2223-2238 0378-1909 98:11<2223 2015 98 10641 https://doi.org/10.1007/s10641-015-0443-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10641-015-0443-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seeley Matthew Marine Science Institute, University of Texas at Austin, 750 Channel View Drive, 78373, Port Aransas, TX, USA aut NATIONALLICENCE 700 1- Miller Nathaniel Jackson School of Geosciences, University of Texas at Austin, 23rd St, 78759, Austin, TX, USA aut NATIONALLICENCE 700 1- Walther Benjamin Marine Science Institute, University of Texas at Austin, 750 Channel View Drive, 78373, Port Aransas, TX, USA aut NATIONALLICENCE 773 0- Environmental Biology of Fishes Springer Netherlands 98/11(2015-11-01), 2223-2238 0378-1909 98:11<2223 2015 98 10641