caa a22 4500 606229434 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0006-9 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0006-9 Effects of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment on ovarian development of the sapphire devil, Chrysiptera cyanea [Elektronische Daten] [Muhammad Badruzzaman, Satoshi Imamura, Yuki Takeuchi, Taro Ikegami, Akihiro Takemura] In the neuroendocrine system controlling fish reproduction, dopamine (DA) acts as a gonadotropin inhibitory factor and plays a role in regulating gonadal development of certain species. The present study examined the effects of chemical destruction of dopaminergic neurons in the brain on DA production and ovarian development in the sapphire devil Chrysiptera cyanea, a reef-associated damselfish. The avidin-biotin-peroxidase complex method using an antibody against tyrosine hydroxylase (TH), a critical enzyme in the DA synthesis pathway, identified a population of dopaminergic neurons with somata in the anteroventral preoptic nucleus of the diencephalon and fibers terminating in the proximal pars distalis of the pituitary. Maintaining fish in seawater containing 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) at 0.02 and 0.2µg/mL for 2days resulted in decreases in DA, 3,4-dihydroxyphenylacetic acid (DOPAC; DA metabolite), and DA metabolic rate in the whole brain. The number of TH-positive neurons in the diencephalon decreased after 0.02µg/mL MPTP treatment for 2days. These results suggest that MPTP treatment destroys TH-positive neurons in the diencephalon, thereby decreasing the synthesis and release of DA from the brain. This treatment rescued ovarian development in fish with artificially retracted ovaries during the spawning season. The gonadosomatic index of MPTP-treated fish 5 and 7days after treatment was significantly higher than that of control fish. Oocytes in the vitellogenic stages were observed in the ovaries of MPTP-treated fish, but not in control fish. These results suggest that DA in the brain drives ovarian development in the sapphire devil. Springer Science+Business Media Dordrecht, 2014 Damselfish nationallicence Dopamine nationallicence MPTP nationallicence Ovary nationallicence Reproduction nationallicence Tyrosine hydroxylase nationallicence Vitellogenesis nationallicence Badruzzaman Muhammad Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut Imamura Satoshi Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut Takeuchi Yuki Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut Ikegami Taro Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut Takemura Akihiro Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 61-71 0920-1742 41:1<61 2015 41 10695 https://doi.org/10.1007/s10695-014-0006-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0006-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Badruzzaman Muhammad Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut NATIONALLICENCE 700 1- Imamura Satoshi Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut NATIONALLICENCE 700 1- Takeuchi Yuki Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut NATIONALLICENCE 700 1- Ikegami Taro Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut NATIONALLICENCE 700 1- Takemura Akihiro Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, 903-0213, Nishihara, Okinawa, Japan aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 61-71 0920-1742 41:1<61 2015 41 10695