caa a22 4500 606229442 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0007-8 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0007-8 Conjugated linoleic acid alters growth performance, tissue lipid deposition, and fatty acid composition of darkbarbel catfish ( Pelteobagrus vachelli ) [Elektronische Daten] [Gui-Fang Dong, Wen-Zuo Liu, Lin-Zhou Wu, Deng-hang Yu, Feng Huang, Peng-Cheng Li, Yan-ou Yang] Fatty liver syndrome is a prevalent problem of farmed fish. Conjugated linoleic acid (CLA) has received increased attention recently as a fat-reducing fatty acid to control fat deposition in mammals. Therefore, the aim of the present study was to determine whether dietary CLA can reduce tissue lipid content of darkbarbel catfish (Pelteobagrus vachelli) and whether decreased lipid content is partially due to alterations in lipid metabolism enzyme activities and fatty acid profiles. A 76-day feeding trial was conducted to investigate the effect of dietary CLA on the growth, tissue lipid deposition, and fatty acid composition of darkbarbel catfish. Five diets containing 0% (control), 0.5% (CLA0.5), 1% (CLA1), 2% (CLA2), and 3% (CLA3) CLA levels were evaluated. Results showed that fish fed with 2-3% CLA diets showed a significantly lower specific growth rate and feed conversion efficiency than those fed with the control diet. Dietary CLA decreased the lipid contents in the liver and intraperitoneal fat with the CLA levels from 1 to 3%. Fish fed with 2-3% CLA diets showed significantly higher lipoprotein lipase and hepatic triacylglycerol lipase activities in liver than those of fish fed with the control, and fish fed with 1-3% CLA diets had significantly higher pancreatic triacylglycerol lipase activities in liver than those of fish fed with the control. Dietary CLA was incorporated into liver, intraperitoneal fat, and muscle lipids, with higher percentages observed in liver compared with other tissues. Liver CLA deposition was at the expense of monounsaturated fatty acids (MUFA). In contrast, CLA deposition appeared to be primarily at the expense of MUFA and n-3 polyunsaturated fatty acids (PUFA) in the intraperitoneal fat, whereas in muscle it was at the expense of n-3 PUFA. Our results suggested that CLA at a 1% dose can reduce liver lipid content without eliciting any negative effect on growth rate in darkbarbel catfish. This lipid-lowering effect could be in part due to an increment in the activity of lipid metabolism enzymes and an extensive interconversion of fatty acids. Although CLA deposition in muscle (0.66-3.19% of total fatty acids) are higher than presented in natural sources of CLA, EPA (C20:5n-3) in fish muscle appears simultaneously expendable, when the fish fed with 2-3% CLA. Springer Science+Business Media Dordrecht, 2014 Conjugated linoleic acid nationallicence Lipid deposition nationallicence Lipid metabolism enzymes nationallicence Fatty acid composition nationallicence Pelteobagrus vachelli nationallicence HTL : Hepatic triacylglycerol lipase nationallicence HUFA : Total highly unsaturated fatty acids nationallicence IPF : Intraperitoneal fat ratio nationallicence LPL : Lipoprotein lipase nationallicence MUFA : Total monounsaturated fatty acids nationallicence PTL : Pancreatic triacylglycerol lipase nationallicence PUFA : Polyunsaturated fatty acids nationallicence SFA : Total saturated fatty acids nationallicence Dong Gui-Fang Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut Liu Wen-Zuo Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut Wu Lin-Zhou Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut Yu Deng-hang Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut Huang Feng Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut Li Peng-Cheng College of Animal Science and Technology, Anhui Agricultural University, 230000, Hefei, Anhui, China aut Yang Yan-ou College of Animal Science and Technology, Anhui Agricultural University, 230000, Hefei, Anhui, China aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 73-89 0920-1742 41:1<73 2015 41 10695 https://doi.org/10.1007/s10695-014-0007-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0007-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Gui-Fang Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Liu Wen-Zuo Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Wu Lin-Zhou Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Yu Deng-hang Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Huang Feng Hubei Key Laboratory of Animal Nutrition and Feed Science, 430023, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Li Peng-Cheng College of Animal Science and Technology, Anhui Agricultural University, 230000, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Yang Yan-ou College of Animal Science and Technology, Anhui Agricultural University, 230000, Hefei, Anhui, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 73-89 0920-1742 41:1<73 2015 41 10695