caa a22 4500 606229450 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0017-6 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0017-6 Effects of fructooligosaccharide on immune response, antioxidant capability and HSP70 and HSP90 expressions of blunt snout bream ( Megalobrama amblycephala ) under high ammonia stress [Elektronische Daten] [Chun-Nuan Zhang, Xiang-Fei Li, Hong-Yan Tian, Ding-Dong Zhang, Guang-Zhen Jiang, Kang-Le Lu, Guang-Xia Liu, Wen-Bin Liu] This study aimed to determine the effects of fructooligosaccharide (FOS) on immune response, antioxidant capability and HSP70 and HSP90 mRNA expressions of blunt snout bream (Megalobrama amblycephala) under high ammonia stress. A total of 360 fish were randomly distributed into three groups (each with four replicates) and were fed three levels of FOS (0, 0.4 and 0.8%) for 8weeks. After the feeding trial, 24 fish per tank were exposed to ammonia at 10mgL−1. After stress, plasma cortisol and glucose levels of fish fed 0.4% FOS were all significantly lower than that of the control group at 6 and 3h, respectively. Plasma lysozyme and alternative complement pathway (ACH50) activities as well as nitrogen monoxide (NO) levels all increased significantly with the maximum levels being attained at 6, 6 and 3h, respectively. Thereafter, these parameters all decreased significantly. In addition, fish fed 0.4% FOS showed higher immune parameters under stress compared with that of control group. In addition, liver superoxide dismutase and catalase activities of fish fed 0.4% FOS were both significantly higher than that of the control group before and after stress, while the opposite was true for malondialdehyde content. After stress, the expression of HSP70 and HSP90 of fish fed FOS was significantly higher than that of the control group at 6 and 12h, respectively. After 12h stress, the cumulative mortality of fish fed FOS was significantly lower than that of the control. The results indicated that the supplementation of 0.4% FOS could increase the nonspecific immunity, antioxidant capacity and HSP70 and HSP90 expression of blunt snout bream and enhance its resistance to high ammonia stress. Springer Science+Business Media Dordrecht, 2014 Immunostimulant nationallicence Fish nationallicence Oxidative stress nationallicence Heat-shock proteins nationallicence Ammonium chloride nationallicence Zhang Chun-Nuan Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Li Xiang-Fei Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Tian Hong-Yan Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Zhang Ding-Dong Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Jiang Guang-Zhen Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Lu Kang-Le Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Liu Guang-Xia Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Liu Wen-Bin Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 203-217 0920-1742 41:1<203 2015 41 10695 https://doi.org/10.1007/s10695-014-0017-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0017-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Chun-Nuan Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Li Xiang-Fei Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Tian Hong-Yan Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Ding-Dong Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Guang-Zhen Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Lu Kang-Le Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Liu Guang-Xia Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Liu Wen-Bin Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 203-217 0920-1742 41:1<203 2015 41 10695