caa a22 4500 606229485 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0024-7 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0024-7 β-Alanine does not act through branched-chain amino acid catabolism in carp, a species with low muscular carnosine storage [Elektronische Daten] [F. Geda, A. Declercq, A. Decostere, A. Lauwaerts, B. Wuyts, W. Derave, G. Janssens] This study was executed to investigate the effect of dietary β-alanine (BA) on amino acid (AA) metabolism and voluntary feed intake in carp (Cyprinus carpio) at mildly elevated temperature to exert AA catabolism. Twenty-four fish in 12 aquaria were randomly assigned to either a control diet or the same diet with 500mgBA/kg. A 14-day period at an ideal temperature (23°C) was followed by 15days at chronic mildly elevated temperature (27°C). After the 15days, all fish were euthanised for muscle analysis on histidine-containing dipeptides (HCD), whole blood on free AA and carnitine esters. The carnosine and anserine analysis indicated that all analyses were below the detection limit of 5µmol/L, confirming that carp belongs to a species that does not store HCD. The increases in free AA concentrations due to BA supplementation failed to reach the level of significance. The effects of dietary BA on selected whole blood carnitine esters and their ratios were also not significant. The supplementation of BA tended to increase body weight gain (P=0.081) and feed intake (P=0.092). The lack of differences in the selected nutrient metabolites in combination with tendencies of improved growth performance warrants further investigation to unravel the mechanism of BA affecting feed intake. This first trial on the effect of BA supplementation on AA catabolism showed that its metabolic effect in carp at chronic mildly elevated temperature was very limited. Further studies need to evaluate which conditions are able to exert an effect of BA on AA metabolism. Springer Science+Business Media Dordrecht, 2014 Carp nationallicence Elevated temperature nationallicence β-Alanine nationallicence Amino acids nationallicence Acylcarnitine nationallicence AA : Amino acids nationallicence BA : β-Alanine nationallicence BCAA : Branched-chain amino acids nationallicence CON : Control nationallicence FCR : Feed conversion ratio nationallicence GABA : γ-Aminobutyric acid nationallicence HCD : Histidine-containing dipeptides nationallicence HPLC : High-performance liquid chromatography nationallicence SEM : Standard error of mean nationallicence TAC : Total acylcarnitine nationallicence $$\sum$$ ∑ 3OH-FA : Sum of 3-hydroxybutyryl fatty acids nationallicence $$\sum$$ ∑ FA : Sum of 3-hydroxybutyryl respective fatty acids nationallicence Geda F. Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820, Merelbeke, Belgium aut Declercq A. Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium aut Decostere A. Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium aut Lauwaerts A. Taminco BVBA, Pantserschipstraat 207, 9000, Ghent, Belgium aut Wuyts B. Department of Clinical Chemistry, University Hospital Ghent, De Pintelaan 185, 9000, Ghent, Belgium aut Derave W. Department of Movement and Sports Sciences, Faculty of Medicine and Health Sciences, Ghent University, Watersportlaan 2, 9000, Ghent, Belgium aut Janssens G. Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820, Merelbeke, Belgium aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 281-287 0920-1742 41:1<281 2015 41 10695 https://doi.org/10.1007/s10695-014-0024-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0024-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Geda F. Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820, Merelbeke, Belgium aut NATIONALLICENCE 700 1- Declercq A. Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium aut NATIONALLICENCE 700 1- Decostere A. Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium aut NATIONALLICENCE 700 1- Lauwaerts A. Taminco BVBA, Pantserschipstraat 207, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Wuyts B. Department of Clinical Chemistry, University Hospital Ghent, De Pintelaan 185, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Derave W. Department of Movement and Sports Sciences, Faculty of Medicine and Health Sciences, Ghent University, Watersportlaan 2, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Janssens G. Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820, Merelbeke, Belgium aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 281-287 0920-1742 41:1<281 2015 41 10695