caa a22 4500 606229493 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0015-8 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0015-8 cDNA structure and the effect of fasting on myostatin expression in walking catfish ( Clarias macrocephalus , Günther 1864) [Elektronische Daten] [Poonmanee Kanjanaworakul, Prapansak Srisapoome, Orathai Sawatdichaikul, Supawadee Poompuang] We cloned and sequenced the myostatin (MSTN) gene of walking catfish and characterized its expression under different conditions. The full cDNA sequence of MSTN was 1,784bp, containing an open reading frame of 1,191bp, which encoded 396 amino acids. The deduced MSTN sequence contained functional sites similar to other members of TGF-β superfamily, including the proteolytic processing site and nine conserved cysteines in the C-terminal. Walking catfish MSTN mRNA was strongly expressed in skeletal muscle and brain tissues, consistent with the expression profiles of MSTN-1 isoform in other teleosts. Temporal expression analysis revealed that the MSTN was expressed at the highest levels in 1-week-old larvae and adults, but was lowest in early juveniles. A fasting-re-feeding experiment was used to evaluate the effects of starvation on growth and MSTN expression in juvenile walking catfish for 28days. MSTN transcript levels increased significantly (threefold) after 7days of fasting (P<0.05) compared with the fed control. Subsequently, MSTN expression levels decreased 1.6-fold when fasting was extended to 14days. Although re-feeding decreased the MSTN expression relative to the levels of the fed control, the period was not long enough for growth recovery of the juveniles. Our results supported a role of MSTN as a negative regulator of muscle growth and, possibly, a role in energy conservation in fish. Springer Science+Business Media Dordrecht, 2014 Clarias macrocephalus nationallicence Myostatin nationallicence cDNA cloning nationallicence Muscle growth nationallicence Gene expression nationallicence Kanjanaworakul Poonmanee Center for Agricultural Biotechnology, Kasetsart University, 73140, Nakhon Pathom, Thailand aut Srisapoome Prapansak Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Ngamwongwan Road, 10900, Bangkok, Thailand aut Sawatdichaikul Orathai Department of Nutrition and Health, Institute of Food Research and Product Development, Kasetsart University, 10900, Bangkok, Thailand aut Poompuang Supawadee Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Ngamwongwan Road, 10900, Bangkok, Thailand aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 177-191 0920-1742 41:1<177 2015 41 10695 https://doi.org/10.1007/s10695-014-0015-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0015-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanjanaworakul Poonmanee Center for Agricultural Biotechnology, Kasetsart University, 73140, Nakhon Pathom, Thailand aut NATIONALLICENCE 700 1- Srisapoome Prapansak Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Ngamwongwan Road, 10900, Bangkok, Thailand aut NATIONALLICENCE 700 1- Sawatdichaikul Orathai Department of Nutrition and Health, Institute of Food Research and Product Development, Kasetsart University, 10900, Bangkok, Thailand aut NATIONALLICENCE 700 1- Poompuang Supawadee Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Ngamwongwan Road, 10900, Bangkok, Thailand aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 177-191 0920-1742 41:1<177 2015 41 10695