caa a22 4500 606229507 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0016-7 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0016-7 Methyl glycol, methanol and DMSO effects on post-thaw motility, velocities, membrane integrity and mitochondrial function of Brycon orbignyanus and Prochilodus lineatus (Characiformes) sperm [Elektronische Daten] [Ana Viveiros, Ariane Nascimento, Marcelo Leal, Antônio Gonçalves, Laura Orfão, Jacky Cosson] The aim of this study was to use more accurate techniques to investigate the effects of cryoprotectants (CPAs) and extenders on post-thaw sperm quality of Brycon orbignyanus and Prochilodus lineatus. Six freezing media comprising the combination of three CPAs (DMSO, methanol and methyl glycol) and two extenders (BTS and glucose) were used. Sperm was diluted in each medium, loaded into 0.5-mL straws, frozen in a nitrogen vapor vessel (dry-shipper), and stored in liquid nitrogen at −196°C. Post-thaw sperm motility rate and velocities (curvilinear=VCL; straight line=VSL; average path=VAP) were evaluated using a computer-assisted sperm analyzer. Membrane integrity and mitochondrial function were determined using fluorochromes. Post-thaw quality was considered high when samples presented the following minimum values: 60% motile sperm, 140µm/s of VCL, 50% intact sperm membrane and 50% mitochondrial function integrity. High post-thaw quality was observed in B. orbignyanus sperm frozen in BTS-methyl glycol and in P. lineatus sperm frozen in BTS-methyl glycol, glucose-methyl glycol and glucose-methanol. All samples frozen in DMSO yielded low quality. The presence of ions in the BTS extender affected post-thaw sperm quality positively in B. orbignyanus and negatively in P. lineatus. Methyl glycol was the most suitable CPA for both fish species, leading to a good protection of cell membrane, mitochondrial function and motility apparatus during the cryopreservation process. For an improved protection, B. orbignyanus sperm should be frozen in an ionic freezing medium. Springer Science+Business Media Dordrecht, 2014 Cryoprotection nationallicence CASA nationallicence Semen nationallicence Neotropical nationallicence Teleost nationallicence Fish nationallicence Viveiros Ana Department of Animal Science (DZO), Federal University of Lavras (UFLA), 37200-000, Lavras, MG, Brazil aut Nascimento Ariane Department of Animal Science (DZO), Federal University of Lavras (UFLA), 37200-000, Lavras, MG, Brazil aut Leal Marcelo Department of Animal Science (DZO), Federal University of Lavras (UFLA), 37200-000, Lavras, MG, Brazil aut Gonçalves Antônio Department of Animal Science (DZO), Federal University of Lavras (UFLA), 37200-000, Lavras, MG, Brazil aut Orfão Laura Department of Agronomy, José do Rosário Vellano University (UNIFENAS), 37130-000, Alfenas, MG, Brazil aut Cosson Jacky Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, 38925, Vodnany, Czech Republic aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 193-201 0920-1742 41:1<193 2015 41 10695 https://doi.org/10.1007/s10695-014-0016-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0016-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Viveiros Ana Department of Animal Science (DZO), Federal University of Lavras (UFLA), 37200-000, Lavras, MG, Brazil aut NATIONALLICENCE 700 1- Nascimento Ariane Department of Animal Science (DZO), Federal University of Lavras (UFLA), 37200-000, Lavras, MG, Brazil aut NATIONALLICENCE 700 1- Leal Marcelo Department of Animal Science (DZO), Federal University of Lavras (UFLA), 37200-000, Lavras, MG, Brazil aut NATIONALLICENCE 700 1- Gonçalves Antônio Department of Animal Science (DZO), Federal University of Lavras (UFLA), 37200-000, Lavras, MG, Brazil aut NATIONALLICENCE 700 1- Orfão Laura Department of Agronomy, José do Rosário Vellano University (UNIFENAS), 37130-000, Alfenas, MG, Brazil aut NATIONALLICENCE 700 1- Cosson Jacky Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, 38925, Vodnany, Czech Republic aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 193-201 0920-1742 41:1<193 2015 41 10695