caa a22 4500 606229515 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0008-7 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0008-7 Alterations of digestive enzyme activities, intestinal morphology and microbiota in juvenile paddlefish, Polyodon spathula , fed dietary probiotics [Elektronische Daten] [Cheng Fang, Mingyang Ma, Hong Ji, Tongjun Ren, Steven Mims] The effects of dietary supplementation of probiotics on digestive enzymes activities, intestinal morphology and microbiota in juvenile paddlefish (Polyodon spathula) were studied. A total of 400 fish were reared in two cages and fed with a basal diet (control group, CG) or diet supplemented with commercial probiotics (treatment group, TG) for 80days. Enzymes activities analysis indicated that protease and α-amylase activities increased (P<0.01 or P<0.05) in TG. Light microscopy observation demonstrated the decrease of wall thickness and muscularis thickness in foregut (P<0.01), the increase of those in hindgut (P<0.05), the increase of folds height in foregut (P<0.01) and midgut in TG (P<0.05). DGGE results of PCR-amplified 16S rRNA confirmed that the richness and diversity of intestinal microbial species increased in TG. The similarity between the commercial bacteria product and intestinal microbiota of TG were higher than the microbiota from CG. The quantities of bacterium, Firmicutes, Proteobacteria, Bacteroidetes, Fusobacteria, present an increasing trend from foregut to hindgut both in two groups. To our knowledge, this is the first in vivo study to reveal the effect of dietary probiotics on intestinal digestive enzymes activities, morphology and microbiota in paddlefish. Springer Science+Business Media Dordrecht, 2014 Probiotics nationallicence Paddlefish nationallicence Digestive enzyme nationallicence Intestinal morphology nationallicence Intestinal microbiota nationallicence Fang Cheng College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut Ma Mingyang College of Innovation Experiment, Northwest A&F University, 712100, Yangling, People's Republic of China aut Ji Hong College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut Ren Tongjun College of Fisheries and Life Science, Dalian Ocean University, 116023, Dalian, People's Republic of China aut Mims Steven Aquaculture Research Center, Kentucky State University, 40601, Frankfort, KY, USA aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 91-105 0920-1742 41:1<91 2015 41 10695 https://doi.org/10.1007/s10695-014-0008-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0008-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fang Cheng College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 700 1- Ma Mingyang College of Innovation Experiment, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 700 1- Ji Hong College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 700 1- Ren Tongjun College of Fisheries and Life Science, Dalian Ocean University, 116023, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Mims Steven Aquaculture Research Center, Kentucky State University, 40601, Frankfort, KY, USA aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 91-105 0920-1742 41:1<91 2015 41 10695