caa a22 4500 606229523 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0013-x doi (NATIONALLICENCE)springer-10.1007/s10695-014-0013-x Hepatoprotective effects of a Chinese herbal formulation, Yingchen decoction, on olaquindox-induced hepatopancreas injury in Jian carp ( Cyprinus carpio var. Jian ) [Elektronische Daten] [Jishu Zhou, Chao Li, Lihong Wang, Hong Ji, Tianhe Zhu] In order to identify effective hepatoprotective herbs for clinical application in fish farming, 200mg/kg olaquindox (OLA) was added to a basal diet (group 1, control) to form OLA diet (group 2), then 1.35, 2.7 and 5.4% (w/w) of a Chinese herbal formulation, Yingchen decoction (YCD), were added to the OLA diet to form three additional diets for groups 3, 4 and 5, respectively. A total of 375 juvenile Jian carp (Cyprinus carpio var. Jian) (52.12±2.95g/tail) were divided into five groups (triplicates per group) and fed the five diets mentioned above, respectively, for 6weeks. At the termination of feeding experiment, serum biochemical indexes, viability of hepatocytes and the hepatopancreas microstructure for each group were detected and observed. The results showed that serum ALT and AST in group 2 were significantly higher than the control (P<0.05). Plasma membranes hepatocyte nuclei in group 2 were found to be mostly indistinct, compared to group 1, and gradually recovered with the increasing supplementation of YCD in group 3, 4 and 5. The viability of isolated hepatocytes in group 2 was the lowest and gradually recovered with the increasing supplementation of YCD in group 3, 4 and 5. The results suggest that YCD protected the Jian carp hepatopancreas against injury from OLA, and that 5.4% YCD would be the optimum dosage in a Jian carp diet. Springer Science+Business Media Dordrecht, 2014 Hepatoprotective nationallicence Chinese herbal formulation nationallicence Yingchen decoction (YCD) nationallicence Olaquindox (OLA) nationallicence Hepatopancreas injury nationallicence Jian carp ( Cyprinus carpio var. Jian ) nationallicence Zhou Jishu Department of Fisheries Science, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut Li Chao Station of China Fisheries Law Enforcement, 71200, Xi'an, Shaanxi, People's Republic of China aut Wang Lihong Nutrition Source Institute of Beijing, Beijing Sunpu Biochemical and Technology Co., Ltd., 100069, Beijing, People's Republic of China aut Ji Hong Department of Fisheries Science, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut Zhu Tianhe Shaanxi Hua Qin Farming Technology Co., Ltd., 712100, Yangling, Shaanxi, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 153-163 0920-1742 41:1<153 2015 41 10695 https://doi.org/10.1007/s10695-014-0013-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0013-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Jishu Department of Fisheries Science, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Li Chao Station of China Fisheries Law Enforcement, 71200, Xi'an, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Wang Lihong Nutrition Source Institute of Beijing, Beijing Sunpu Biochemical and Technology Co., Ltd., 100069, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Ji Hong Department of Fisheries Science, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Tianhe Shaanxi Hua Qin Farming Technology Co., Ltd., 712100, Yangling, Shaanxi, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 153-163 0920-1742 41:1<153 2015 41 10695