caa a22 4500 60622954X CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0005-x doi (NATIONALLICENCE)springer-10.1007/s10695-014-0005-x GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus [Elektronische Daten] [Tomás Delgadin, Daniela Pérez Sirkin, María Di Yorio, Silvia Arranz, Paula Vissio] Cichlasoma dimerus is a social cichlid fish capable of growing at high rates under laboratory conditions, but knowledge on somatic growth regulation is still unclear. Growth hormone (GH)/insulin-like growth factor I (IGF-I) axis is the key regulator of somatic growth in vertebrates. Two types of growth hormone receptors have been described in teleost fish, named GH receptor type 1 (GHR1) and type 2 (GHR2). In addition, isoforms of these receptors lacking part of the intracellular region have been described. The aim of this study was to evaluate the somatic growth, liver histology and changes in the GH/IGF-I axis after 4weeks of food deprivation in C. dimerus. Four-week fasted fish showed reductions in specific growth rates in body weight (p<0.001) and standard length (p<0.001). Additionally, the hepatosomatic index (p<0.001) and hepatocyte area (p<0.001) decreased in fasted fish, while no changes in glucose levels were detected in plasma. The starvation protocol failed to induce changes in GH mRNA levels in the pituitary and IGF-I mRNA levels in liver. In contrast, IGF-I mRNA levels in muscle decreased in fasted fish (p=0.002). On the other hand, GHR2 (detected with primer sets designed over the extracellular and intracellular region) was upregulated by starvation both in liver and muscle (p<0.05), while GHR1 remained unchanged. These results show that a fasting period reduced somatic growth both in length and body weight concomitantly with alterations on liver and muscle GHR2 and muscle IGF-I mRNA expression. Springer Science+Business Media Dordrecht, 2014 Growth hormone nationallicence GHR1 nationallicence GHR2 nationallicence IGF-I nationallicence Cichlid nationallicence Delgadin Tomás Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina aut Pérez Sirkin Daniela Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina aut Di Yorio María Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina aut Arranz Silvia Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET)-Área Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, CONICET and Universidad Nacional de Rosario, Ocampo y Esmeralda s/n, Rosario (2000), Provincia de Santa Fe, Argentina aut Vissio Paula Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 51-60 0920-1742 41:1<51 2015 41 10695 https://doi.org/10.1007/s10695-014-0005-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0005-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Delgadin Tomás Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Pérez Sirkin Daniela Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Di Yorio María Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Arranz Silvia Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET)-Área Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, CONICET and Universidad Nacional de Rosario, Ocampo y Esmeralda s/n, Rosario (2000), Provincia de Santa Fe, Argentina aut NATIONALLICENCE 700 1- Vissio Paula Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 51-60 0920-1742 41:1<51 2015 41 10695