caa a22 4500 606229558 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-9983-y doi (NATIONALLICENCE)springer-10.1007/s10695-014-9983-y Metabolic effects of trichlorfon (Masoten®) on the neotropical freshwater fish pacu ( Piaractus mesopotamicus ) [Elektronische Daten] [Francine Venturini, Fernanda Moraes, Lucas Cortella, Priscila Rossi, Claudinei Cruz, Gilberto Moraes] Fish parasites are among the crucial limiting factors in aquaculture. The organophosphorous pesticide trichlorfon is widely used as an insecticide and against fish parasites worldwide. In this study, the effects of environmental trichlorfon on biochemical and physiological parameters were investigated in Piaractus mesopotamicus (pacu), a widely farmed fish in South America, through sublethal exposure (8µgL−1, 10% of the LC50; 96h) and recovery. The activity of brain acetylcholinesterase (AChE) was reduced after exposure (15.5%) and remained decreased during the recovery (21.5%). In white muscle, AChE activity decreased 31% only after recovery. Alkaline phosphatase (ALP) and acid phosphatase (ACP) activities of the liver, muscle and plasma were steady during exposure. However, after the recovery period, ALP activity was increased in the liver and muscle and decreased in plasma, while ACP was increased in the liver and decreased in muscle. Intermediary metabolism was also affected by trichlorfon, depicting increase of energetic demand (hypoglycemia, neoglucogenesis and lipid catabolism), which remained even after recovery. These results indicate that P. mesopotamicus is adversely affected by sublethal concentrations of trichlorfon and are useful for assessing the impact as well as the pros and cons of its use in controlling fish parasites in aquaculture. Springer Science+Business Media Dordrecht, 2014 Organophosphorus nationallicence AChE nationallicence Phosphatases nationallicence Metabolism nationallicence Freshwater fish nationallicence Venturini Francine Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut Moraes Fernanda Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut Cortella Lucas Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut Rossi Priscila Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut Cruz Claudinei Weed Environmental Research and Studies at UNESP (NEPEAM), Rod. Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP, Brazil aut Moraes Gilberto Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 299-309 0920-1742 41:1<299 2015 41 10695 https://doi.org/10.1007/s10695-014-9983-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-9983-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Venturini Francine Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Moraes Fernanda Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Cortella Lucas Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Rossi Priscila Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Cruz Claudinei Weed Environmental Research and Studies at UNESP (NEPEAM), Rod. Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP, Brazil aut NATIONALLICENCE 700 1- Moraes Gilberto Department of Genetics and Evolution, Federal University of Sao Carlos, Rod. Washington Luiz Km 235, CEP 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 299-309 0920-1742 41:1<299 2015 41 10695