caa a22 4500 606229582 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-9984-x doi (NATIONALLICENCE)springer-10.1007/s10695-014-9984-x Hematological changes and cytogenotoxicity in the tilapia Oreochromis niloticus caused by sub-chronic exposures to mercury and selenium [Elektronische Daten] [Robson Seriani, Jakeline França, Julio Lombardi, Jôse Brito, Maria Ranzani-Paiva] Fish bioassays are valuable tools that can be used to elucidate the toxicological potential of numerous substances that are present in the aquatic environment. In this study, we assessed the antagonistic action of selenium (Se) against the toxicity of mercury (Hg) in fish (Oreochromis niloticus). Six experimental groups with six fish each were defined as follows: (1) control, (2) mercury (HgCl2), (3) sodium selenite (Na2Se4O3), (4) sodium selenate (Na2Se6O4), (5) mercury+sodium selenite (HgCl2+Na2Se4O3), and (6) mercury+sodium selenate (HgCl2+Na2Se6O4). Hematological parameters [red blood cells (RBC), white blood cells (WBC), and erythroblasts (ERB)] in combination with cytogenotoxicity biomarkers [nuclear abnormalities (NAs) and micronuclei (MN)] were examined after three, seven, ten, and fourteen days. After 7days of exposure, cytogenotoxic effects and increased erythroblasts caused by mercury, leukocytosis triggered by mercury+sodium selenite, leukopenia associated with sodium selenate, and anemia triggered by mercury+sodium selenate were observed. Positive correlations that were independent of time were observed between WBC and RBC, ERB and MN, and NA and MN. The results suggest that short-term exposure to chemical contaminants elicited changes in blood parameters and produced cytogenotoxic effects. Moreover, NAs are the primary manifestations of MN formation and should be included in a class characterized as NA only. Lastly, the staining techniques used can be applied to both hematological characterization and the measurement of cytogenotoxicity biomarkers. Springer Science+Business Media Dordrecht, 2014 Toxicity nationallicence Mercury nationallicence Selenium nationallicence Hematology nationallicence Nuclear abnormalities nationallicence Micronuclei nationallicence Seriani Robson Centro de Aquicultura, Instituto de Pesca, Avenida Francisco Matarazzo, 455, 05001-900, São Paulo, SP, Brazil aut França Jakeline Centro de Aquicultura, Campus Jaboticabal, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brazil aut Lombardi Julio Centro de Aquicultura, Instituto de Pesca, Avenida Francisco Matarazzo, 455, 05001-900, São Paulo, SP, Brazil aut Brito Jôse Faculdade de Medicina, Universidade de São Paulo, Avenida Dr. Arnaldo, 455-room 1150, 01246-903, São Paulo, SP, Brazil aut Ranzani-Paiva Maria Centro de Aquicultura, Instituto de Pesca, Avenida Francisco Matarazzo, 455, 05001-900, São Paulo, SP, Brazil aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 311-322 0920-1742 41:1<311 2015 41 10695 https://doi.org/10.1007/s10695-014-9984-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-9984-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seriani Robson Centro de Aquicultura, Instituto de Pesca, Avenida Francisco Matarazzo, 455, 05001-900, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- França Jakeline Centro de Aquicultura, Campus Jaboticabal, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brazil aut NATIONALLICENCE 700 1- Lombardi Julio Centro de Aquicultura, Instituto de Pesca, Avenida Francisco Matarazzo, 455, 05001-900, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Brito Jôse Faculdade de Medicina, Universidade de São Paulo, Avenida Dr. Arnaldo, 455-room 1150, 01246-903, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Ranzani-Paiva Maria Centro de Aquicultura, Instituto de Pesca, Avenida Francisco Matarazzo, 455, 05001-900, São Paulo, SP, Brazil aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 311-322 0920-1742 41:1<311 2015 41 10695