caa a22 4500 606229590 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0001-1 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0001-1 Dietary lipid levels impact lipoprotein lipase, hormone-sensitive lipase, and fatty acid synthetase gene expression in three tissues of adult GIFT strain of Nile tilapia, Oreochromis niloticus [Elektronische Daten] [Juan Tian, Fan Wu, Chang-Geng Yang, Ming Jiang, Wei Liu, Hua Wen] The objective of this study was to assess the effects of dietary lipids on growth performance, body composition, serum parameters, and expression of genes involved in lipid metabolism in adult genetically improved farmed tilapia (GIFT strain) of Nile tilapia, Oreochromis niloticus. We randomly assigned adult male Nile tilapia (average initial body weight=220.00±9.54g) into six groups consisting of four replicates (20 fish per replicate). Fish in each group were hand-fed a semi-purified diets containing different lipid levels [3.3 (the control group), 28.4, 51.4, 75.4, 101.9, and 124.1gkg−1] for 8weeks. The results indicated that there was no obvious effect in feeding rate among all groups (P>0.05). The highest weight gain, specific growth rate, and protein efficiency ratio in 75.4gkg−1 diet group were increased by 23.31, 16.17, and 22.02% than that of fish in the control group (P<0.05). Protein retention ratio was highest in 51.4gkg−1 diet group. The results revealed that the optimum dietary lipid level for maximum growth performance is 76.6-87.9gkg−1. Increasing dietary lipid levels contributed to increased tissue and whole body lipid levels. Saturated and monounsaturated fatty acids (MUFAs) decreased, and polyunsaturated fatty acids increased with increasing dietary lipid levels. With the exception of MUFAs, the fatty acid profiles of liver and muscle were similar. Dietary lipid levels were negatively correlated with low-density lipoprotein- cholesterol content and positively with triacylglycerol and glucose contents. In the lipid-fed groups, there was a significant down-regulation of fatty acid synthase (FAS) mRNA in liver, muscle, and visceral adipose tissues. There was a rapid up-regulation of lipoprotein lipase (LPL) mRNA in muscle and liver with increasing dietary lipid levels. In visceral adipose tissue, LPL mRNA was significantly down-regulated in the lipid-fed groups. Dietary lipids increased hormone-sensitive lipase (HSL) mRNA expression levels in the three tissues. These results strongly suggested that moderate dietary lipid levels were beneficial for adult tilapia growth performance and feed efficiency. However, excessive dietary lipid levels contributed to lipid deposition. Additionally, excessive dietary lipids may induce a competition between lipolysis and lipogenesis. FAS did not have tissue-specific regulation; however, the regulation of dietary lipids on LPL expression is tissue specific. FAS was a negative feedback regulator on fat deposition, and HSL was an indicator of fat content in tilapia. Springer Science+Business Media Dordrecht, 2014 Dietary lipid level nationallicence Nile tilapia adult nationallicence Lipid metabolic enzymes nationallicence Gene expression nationallicence Tian Juan Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut Wu Fan Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut Yang Chang-Geng Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut Jiang Ming Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut Liu Wei Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut Wen Hua Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 1-18 0920-1742 41:1<1 2015 41 10695 https://doi.org/10.1007/s10695-014-0001-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0001-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tian Juan Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Wu Fan Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Yang Chang-Geng Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Jiang Ming Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Liu Wei Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Wen Hua Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, 430223, Wuhan, Hubei, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 1-18 0920-1742 41:1<1 2015 41 10695