caa a22 4500 606229604 CHVBK 20210128101209.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0021-x doi (NATIONALLICENCE)springer-10.1007/s10695-014-0021-x Estrogen and glucocorticoid receptor agonists and antagonists in oocytes modulate the pattern of expression of genes that encode nuclear receptor proteins in very early stage rainbow trout ( Oncorhynchus mykiss ) embryos [Elektronische Daten] [Jacqueline Ferris, Mao Li, John Leatherland, W. King] Previous studies show that changes in estrogen (ER) and glucocorticoid receptor (GR) function in rainbow trout (Oncorhynchus mykiss) oocytes modulate the growth performance phenotype of embryo and juvenile progeny; the present study was undertaken to determine whether this altered growth performance is associated with changes in the expression of several growth-related genes in early-stage embryos. Unfertilized oocytes were incubated in the presence of various combinations of GR and ER agonists and antagonists; the oocytes were then fertilized and the expression of genes that encode for six nuclear receptor superfamily (NRS) proteins (GR1, GR2, ERα, ERβ, TRα, and TRβ) and the two IGF peptides (IGF1 and IGF2) were measured in the 7-, 13-, and 26-dpf embryos. By day 26 of embryogenesis, the expression of the six NRS-related genes of interest and that of igf2 were significantly enhanced in embryos reared from ER agonist- or ER antagonist-treated oocytes, regardless of whether the GR agonist, cortisol, was also included in the initial oocyte incubation medium. Conversely, the igf1 expression pattern among treatment groups was significantly enhanced in the cortisol-only treatment group and in the ER antagonist and GR antagonist groups that were co-incubated with cortisol. Additionally, in the ER agonist treatment groups igf1 expression was significantly inhibited when cortisol was included in the oocyte incubation medium. The findings show that a single in ovo exposure to the receptor agonists/antagonists markedly changed the programming of the expression of NRS-related and IGF-related genes of the early-stage trout embryos. Springer Science+Business Media Dordrecht, 2014 Embryogenesis nationallicence igf nationallicence gr nationallicence tr nationallicence er nationallicence Ferris Jacqueline Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada aut Li Mao Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada aut Leatherland John Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada aut King W. Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 255-265 0920-1742 41:1<255 2015 41 10695 https://doi.org/10.1007/s10695-014-0021-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0021-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferris Jacqueline Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Li Mao Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Leatherland John Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada aut NATIONALLICENCE 700 1- King W. Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 255-265 0920-1742 41:1<255 2015 41 10695