caa a22 4500 606229612 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0003-z doi (NATIONALLICENCE)springer-10.1007/s10695-014-0003-z In silico and in vivo analysis of binding affinity of estrogens with estrogen receptor alpha in Channa punctatus (Bloch) [Elektronische Daten] [S. Pipil, V. Kumar, V. Rawat, L. Sharma, N. Sehgal] In the present study, potential interaction between natural estrogens i.e., estrone (E1), estradiol (E2) and estriol (E3) with human estrogen receptor (hER) was seen by in silico study. Molecular docking studies were carried out using Glide and ligand docking program. The binding affinity, assessed by Glide score, indicates stronger interaction of E3 with hER followed by E2 and E1. Real-time PCR analysis of vga and vgb expressions, in the liver of different groups of Channa punctatus injected with the three natural estrogens, supported the docking analysis and indicated E3 to be the most potent estrogen in inducing vga and vgb expressions followed by E2 and E1. This study lays the groundwork for studying interactions of various estrogenic substances with different estrogen receptors and to assess estrogenicity of various chemicals which are being released into the environment byemploying molecular docking technique. Springer Science+Business Media Dordrecht, 2014 Estradiol-17β nationallicence Estriol nationallicence Estrone nationallicence Molecular docking nationallicence Real-time PCR nationallicence Pipil S. Department of Zoology, University of Delhi, 110007, Delhi, India aut Kumar V. Department of Zoology, University of Delhi, 110007, Delhi, India aut Rawat V. Department of Zoology, Hindu College, University of Delhi, 110007, Delhi, India aut Sharma L. Department of Zoology, University of Delhi, 110007, Delhi, India aut Sehgal N. Department of Zoology, University of Delhi, 110007, Delhi, India aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 31-40 0920-1742 41:1<31 2015 41 10695 https://doi.org/10.1007/s10695-014-0003-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0003-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pipil S. Department of Zoology, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Kumar V. Department of Zoology, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Rawat V. Department of Zoology, Hindu College, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Sharma L. Department of Zoology, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Sehgal N. Department of Zoology, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 31-40 0920-1742 41:1<31 2015 41 10695