caa a22 4500 606229620 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-9982-z doi (NATIONALLICENCE)springer-10.1007/s10695-014-9982-z Acute effects of β-naphthoflavone on cardiorespiratory function and metabolism in adult zebrafish ( Danio rerio ) [Elektronische Daten] [Courtney Gerger, Jith Thomas, David Janz, Lynn Weber] Aryl hydrocarbon receptor (AhR) agonists are known to cause lethal cardiovascular deformities in fish after developmental exposure. Acute adult fish toxicity of AhR agonists is thought to be minimal, but limited evidence suggests sublethal effects may also involve the cardiac system in fish. In the present study, adult zebrafish (Danio rerio) were aqueously exposed to solvent control or three nominal concentrations of the commonly used model AhR agonist, β-naphthoflavone (BNF), for 48h. Following exposure, fish were subjected to echocardiography to determine cardiac function or swimming tests with concurrent oxygen consumption measurement. Critical swimming speed and standard metabolic rate were not significantly changed, while active metabolic rate decreased with increasing BNF exposure, reaching statistical significance at the highest BNF exposure. Factorial aerobic scope was the most sensitive end-point and was decreased at even lower BNF concentrations, indicating a reduced aerobic capacity after acute AhR agonist exposure in adult fish. The highest BNF concentration caused a significant decrease in cardiac output, while increasing the ratio of atrial to ventricular heart rate (indicating atrioventricular conduction blockade). In conclusion, the effect of acute BNF exposure on zebrafish metabolic capacity and cardiac function is likely to be physiologically important given that fish have a critical need for adequate oxygen to fuel essential survival behaviors such as swimming, growth, and reproduction. Future studies should be directed at examining the effects of other polycyclic aromatic hydrocarbons on fish cardiorespiratory function to determine whether their effects and modes of action are similar to BNF. Springer Science+Business Media Dordrecht, 2014 Acute toxicity nationallicence β-Naphthoflavone nationallicence Cardiac toxicity nationallicence Metabolic rate nationallicence Swim performance nationallicence Zebrafish nationallicence AhR : Aryl hydrocarbon receptor nationallicence BNF : β-Naphthoflavone nationallicence U crit : Critical swimming speed nationallicence MO2 : Oxygen consumption rate nationallicence SMR : Standard metabolic rate nationallicence AMR : Active metabolic rate nationallicence F-AS : Factorial aerobic scope nationallicence U i : Highest velocity maintained for an entire time interval during swim testing nationallicence T i : Time that elapsed at fatigue velocity nationallicence U ii : Increment by which water velocity was increased for each interval during swim testing nationallicence T ii : Time for each swimming velocity interval nationallicence Q : Cardiac output nationallicence f H : Heart rate nationallicence V S : Stroke volume nationallicence Gerger Courtney Toxicology Centre, University of Saskatchewan, S7N 5B3, Saskatoon, SK, Canada aut Thomas Jith Toxicology Centre, University of Saskatchewan, S7N 5B3, Saskatoon, SK, Canada aut Janz David Toxicology Centre, University of Saskatchewan, S7N 5B3, Saskatoon, SK, Canada aut Weber Lynn Toxicology Centre, University of Saskatchewan, S7N 5B3, Saskatoon, SK, Canada aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 289-298 0920-1742 41:1<289 2015 41 10695 https://doi.org/10.1007/s10695-014-9982-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-9982-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gerger Courtney Toxicology Centre, University of Saskatchewan, S7N 5B3, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Thomas Jith Toxicology Centre, University of Saskatchewan, S7N 5B3, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Janz David Toxicology Centre, University of Saskatchewan, S7N 5B3, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Weber Lynn Toxicology Centre, University of Saskatchewan, S7N 5B3, Saskatoon, SK, Canada aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 289-298 0920-1742 41:1<289 2015 41 10695