caa a22 4500 606229639 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0010-0 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0010-0 Effect of dietary incorporation of a multi-strain probiotic on growth performance and health status in rainbow trout ( Oncorhynchus mykiss ) [Elektronische Daten] [Ilias Giannenas, Ioannis Karamaligas, Maritsa Margaroni, Ioannis Pappas, Elisabeth Mayer, Pedro Encarnação, Evdokia Karagouni] Dietary supplementation with a multi-strain probiotic containing Bacillus subtilis, Enterococcus faecium, Pediococcus acidilactici and Lactobacillus reuteri has been examined for its effects on growth performance, intestinal microbiota, non-specific immune response and antioxidant status of rainbow trout. Three groups of sub-adult trout were stocked into experimental tanks. A commercial diet was used as control, while the other two groups received diets supplemented with the multi-strain probiotic at levels of 1 and 5gkg−1 diet. The fish were fed to apparent satiation three times daily for 8weeks. Dietary probiotic at 1gkg−1 diet improved (P<0.05) growth and feed efficiency compared to control diet. Lactic acid bacteria loads were higher in probiotic fed fish at both inclusion levels compared to control; however, Enterobacteriaceae, Coliforms and Aeromonas spp. were similar between groups. Dietary probiotics decreased (P<0.05) malondialdehyde formation on day 0 compared to control fish but not on day 5 of storage. Probiotics also increased (P<0.05) the activity of glutathione-based enzymes. Serum lysozyme levels were similar among dietary treatments. Probiotic supplementation at 1gkg−1 diet reduced serum nitric oxide levels compared to control. In conclusion, dietary probiotics at the level of 1gkg−1 of diet exerted both a growth promoting and antioxidant protective activity. Springer Science+Business Media Dordrecht, 2014 Rainbow trout nationallicence Probiotic nationallicence Antioxidant status nationallicence Intestinal microbiota nationallicence Non-specific immunity nationallicence Giannenas Ilias Laboratory of Nutrition, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut Karamaligas Ioannis Laboratory of Pharmacology, Veterinary Faculty, University of Thessaly, 43100, Karditsa, Greece aut Margaroni Maritsa Laboratory of Cellular Immunology, Hellenic Pasteur Institute, Athens, Greece aut Pappas Ioannis Laboratory of Pharmacology, Veterinary Faculty, University of Thessaly, 43100, Karditsa, Greece aut Mayer Elisabeth Biomin GmbH, Industriestrasse 21, 3130, Herzogenburg, Austria aut Encarnação Pedro Biomin GmbH, Industriestrasse 21, 3130, Herzogenburg, Austria aut Karagouni Evdokia Laboratory of Cellular Immunology, Hellenic Pasteur Institute, Athens, Greece aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 119-128 0920-1742 41:1<119 2015 41 10695 https://doi.org/10.1007/s10695-014-0010-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0010-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giannenas Ilias Laboratory of Nutrition, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut NATIONALLICENCE 700 1- Karamaligas Ioannis Laboratory of Pharmacology, Veterinary Faculty, University of Thessaly, 43100, Karditsa, Greece aut NATIONALLICENCE 700 1- Margaroni Maritsa Laboratory of Cellular Immunology, Hellenic Pasteur Institute, Athens, Greece aut NATIONALLICENCE 700 1- Pappas Ioannis Laboratory of Pharmacology, Veterinary Faculty, University of Thessaly, 43100, Karditsa, Greece aut NATIONALLICENCE 700 1- Mayer Elisabeth Biomin GmbH, Industriestrasse 21, 3130, Herzogenburg, Austria aut NATIONALLICENCE 700 1- Encarnação Pedro Biomin GmbH, Industriestrasse 21, 3130, Herzogenburg, Austria aut NATIONALLICENCE 700 1- Karagouni Evdokia Laboratory of Cellular Immunology, Hellenic Pasteur Institute, Athens, Greece aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 119-128 0920-1742 41:1<119 2015 41 10695