caa a22 4500 606229655 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10695-014-0009-6 doi (NATIONALLICENCE)springer-10.1007/s10695-014-0009-6 Characterization of incomplete vitellogenin (VgC) in the Indian freshwater murrel, Channa punctatus (Bloch) [Elektronische Daten] [S. Pipil, V. Rawat, L. Sharma, N. Sehgal] A novel incomplete vitellogenin (VgC) was purified from the plasma of estradiol-treated male murrel, Channa punctatus, by gel filtration chromatography. The native mass of VgC protein was 180kDa, and it resolved as a single peptide of 100kDa on SDS-PAGE. The peptide on subjecting to matrix-assisted laser desorption/ionization-time of flight produced a peptide mass fingerprint. On tandem mass spectrometry, some of these peptides showed mass to charge (m/z) ratio and amino acid sequence similarity with VgC peptides of other teleosts. Phylogenetic analysis revealed a similarity of murrel VgC with fish species of the order Perciformes. Semi-quantitative RT-PCR assay was developed to study expression of vgc gene at variable levels of estradiol exposure. Presence of VgC in males indicates that fish has been exposed to estrogens; hence, it can be used as a biomarker for estrogenic exposure. Springer Science+Business Media Dordrecht, 2014 Incomplete vitellogenin (VgC) nationallicence MALDI-TOF-MS/MS nationallicence Channa punctatus nationallicence RT-PCR nationallicence Pipil S. Department of Zoology, University of Delhi, 110007, Delhi, India aut Rawat V. Department of Zoology, Hindu College, University of Delhi, 110007, Delhi, India aut Sharma L. Department of Zoology, University of Delhi, 110007, Delhi, India aut Sehgal N. Department of Zoology, University of Delhi, 110007, Delhi, India aut Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 107-117 0920-1742 41:1<107 2015 41 10695 https://doi.org/10.1007/s10695-014-0009-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-0009-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pipil S. Department of Zoology, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Rawat V. Department of Zoology, Hindu College, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Sharma L. Department of Zoology, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Sehgal N. Department of Zoology, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/1(2015-02-01), 107-117 0920-1742 41:1<107 2015 41 10695