caa a22 4500 606229698 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0091-4 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0091-4 Role of peptidases of the intestinal microflora and prey in temperature adaptations of the digestive system in planktivorous and benthivorous fish [Elektronische Daten] [V. Kuz'mina, E. Skvortsova, M. Shalygin, K. Kovalenko] Many fish enzymatic systems possess limited adaptations to low temperature; however, little data are available to judge whether enzymes of fish prey and intestinal microbiota can mitigate this deficiency. In this study, the activity of serine peptidases (casein-lytic, mainly trypsin and hemoglobin-lytic, mainly chymotrypsin) of intestinal mucosa, chyme and intestinal microflora in four species of planktivorous (blue bream) and benthivorous (roach, crucian carp, perch) was investigated across a wide temperature range (0-70°C) to identify adaptations to low temperature. At 0°C, the relative activity of peptidases of intestinal mucosa (<13%) and usually intestinal microflora (5-12.6%) is considerably less than that of chyme peptidases (up to 40% of maximal activity). The level of peptidase relative activity in crucian carp intestinal microflora was 45% of maximal activity. The shape of t°-function curves of chyme peptidase also differs in fish from different biotopes. Fish from the littoral group are characterized by a higher degree of adaptation of chyme casein-lytic peptidases to functioning at low temperatures as compared to fish from the pelagic group. The role of intestinal microbiota and prey peptidases in digestive system adaptations of planktivorous and benthivorous fish to low temperatures is discussed. Springer Science+Business Media Dordrecht, 2015 Peptidases nationallicence Intestine nationallicence Mucosa nationallicence Chyme nationallicence Intestinal microflora nationallicence Planktivorous nationallicence Benthivorous fish nationallicence Kuz'mina V. I.D.Papanin Institute for Biology of Inland Waters RAS, PO Box 152742, Borok, Yaroslavl, Russia aut Skvortsova E. FSBEI HPE Yaroslavl State Agricultural Academy, PO Box 150042, Yaroslavl, Russia aut Shalygin M. I.D.Papanin Institute for Biology of Inland Waters RAS, PO Box 152742, Borok, Yaroslavl, Russia aut Kovalenko K. Natural Resources Research Institute, University of Minnesota Duluth, Miller Trunk Highway, PO Box 5013, Duluth, MN, USA aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1359-1368 0920-1742 41:6<1359 2015 41 10695 https://doi.org/10.1007/s10695-015-0091-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0091-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuz'mina V. I.D.Papanin Institute for Biology of Inland Waters RAS, PO Box 152742, Borok, Yaroslavl, Russia aut NATIONALLICENCE 700 1- Skvortsova E. FSBEI HPE Yaroslavl State Agricultural Academy, PO Box 150042, Yaroslavl, Russia aut NATIONALLICENCE 700 1- Shalygin M. I.D.Papanin Institute for Biology of Inland Waters RAS, PO Box 152742, Borok, Yaroslavl, Russia aut NATIONALLICENCE 700 1- Kovalenko K. Natural Resources Research Institute, University of Minnesota Duluth, Miller Trunk Highway, PO Box 5013, Duluth, MN, USA aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1359-1368 0920-1742 41:6<1359 2015 41 10695