caa a22 4500 606229701 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0105-2 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0105-2 Effect of temperature and diet on wound healing in Atlantic salmon ( Salmo salar L.) [Elektronische Daten] [Linda Jensen, Thomas Wahli, Charles McGurk, Tommy Eriksen, Alex Obach, Rune Waagbø, Ana Handler, Carolina Tafalla] Compromised skin integrity of farmed Atlantic salmon, commonly occurring under low temperature and stressful conditions, has major impacts on animal welfare and economic productivity. Even fish with minimal scale loss and minor wounds can suffer from secondary infections, causing downgrading and mortalities. Wound healing is a complex process, where water temperature and nutrition play key roles. In this study, Atlantic salmon (260g) were held at different water temperatures (4 or 12°C) and fed three different diets for 10weeks, before artificial wounds were inflicted and the wound healing process monitored for 2weeks. The fish were fed either a control diet, a diet supplemented with zinc (Zn) or a diet containing a combination of functional ingredients in addition to Zn. The effect of diet was assessed through subjective and quantitative skin histology and the transcription of skin-associated chemokines. Histology confirmed that wound healing was faster at 12°C. The epidermis was more organised, and image analyses of digitised skin slides showed that fish fed diets with added Zn had a significantly larger area of the epidermis covered by mucous cells in the deeper layers after 2weeks, representing more advanced healing progression. Constitutive levels of the newly described chemokines, herein named CK 11A, B and C, confirmed their preferential expression in skin compared to other tissues. Contrasting modulation profiles at 4 and 12°C were seen for all three chemokines during the wound healing time course, while the Zn-supplemented diets significantly increased the expression of CK 11A and B during the first 24h of the healing phase. Springer Science+Business Media Dordrecht, 2015 Chemokine nationallicence Wound healing nationallicence Atlantic salmon nationallicence Mucous cells nationallicence Quantitative histology nationallicence Jensen Linda Skretting Aquaculture Research Centre, PO Box 48, 4001, Stavanger, Norway aut Wahli Thomas Centre for Fish and Wildlife Health, Vetsuisse Faculty, University of Bern, Bern, Switzerland aut McGurk Charles Skretting Aquaculture Research Centre, PO Box 48, 4001, Stavanger, Norway aut Eriksen Tommy Skretting Aquaculture Research Centre, PO Box 48, 4001, Stavanger, Norway aut Obach Alex Skretting Aquaculture Research Centre, PO Box 48, 4001, Stavanger, Norway aut Waagbø Rune National Institute of Nutrition and Seafood Research (NIFES), Bergen, Norway aut Handler Ana Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain aut Tafalla Carolina Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1527-1543 0920-1742 41:6<1527 2015 41 10695 https://doi.org/10.1007/s10695-015-0105-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0105-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jensen Linda Skretting Aquaculture Research Centre, PO Box 48, 4001, Stavanger, Norway aut NATIONALLICENCE 700 1- Wahli Thomas Centre for Fish and Wildlife Health, Vetsuisse Faculty, University of Bern, Bern, Switzerland aut NATIONALLICENCE 700 1- McGurk Charles Skretting Aquaculture Research Centre, PO Box 48, 4001, Stavanger, Norway aut NATIONALLICENCE 700 1- Eriksen Tommy Skretting Aquaculture Research Centre, PO Box 48, 4001, Stavanger, Norway aut NATIONALLICENCE 700 1- Obach Alex Skretting Aquaculture Research Centre, PO Box 48, 4001, Stavanger, Norway aut NATIONALLICENCE 700 1- Waagbø Rune National Institute of Nutrition and Seafood Research (NIFES), Bergen, Norway aut NATIONALLICENCE 700 1- Handler Ana Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain aut NATIONALLICENCE 700 1- Tafalla Carolina Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1527-1543 0920-1742 41:6<1527 2015 41 10695