caa a22 4500 606229728 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0100-7 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0100-7 Effects of different spawning agents on serum levels of reproductive steroid hormones and cortisol level in adult female Barbus sharpeyi (Gunther, 1874) [Elektronische Daten] [Takavar Mohammadian, Pedram Malekpouri, Mojtaba Zare, Mohammad Zainodini] The question of whether, as hormone therapies, spawning agents differ from each other to induce physiological pathways of gametogenesis and oocyte maturation in fish remains important, because it could modify undesirable changes, regulated by endocrine systems of individual fish. A series of experimental treatments were applied to investigate the underlying mechanism(s) in which female bunnei (Barbus sharpeyi) fish respond differently to hormone therapies. Female broodstocks were injected twice (with 12h interval) by three different treatments namely A, B and C. The treatment A received carp pituitary extract (CPE)+luteinizing hormone-releasing hormone analogs (LHRHα2) (0.5mgCPEkg−1BW for first injection and 2mgCPEkg−1BW+10µgLHRHα2kg−1 for second injection), treatment B received CPE (0.5 and 3.5mgkg−1BW), and treatment C received ovaprim (0.1 and 0.15mlkg−1BW). Blood samples were collected at four different time intervals, including prior to injections, 6h after first injection, 6h after second injection and at the time of spawning, and serum steroid hormones, including testosterone, progesterone and estradiol-17β as well as cortisol, were measured. Results showed significant increases in serum estradiol-17β following all treatments, but the most profound response was found in treatments A and B. Testosterone was higher in larger broodfish than in small-sized broodfish (>1.5 vs. <1.5kg) in all treatments. CPE led to higher concentration of testosterone rather than two other treatments. CPE also increases the progesterone following first injection and approximately remains unchanged till the end of experiment. Change in progesterone level was only significant after second injection of ovaprim as well as after spawning compared with previous time. Linear regression analyses indicated that cortisol had adverse effects on progesterone and testosterone levels of weight group <1.5kg. These results suggest that among inducing agents, applied here, CPE can provide more reasonable response in reproduction of female B. sharpeyi. Springer Science+Business Media Dordrecht, 2015 Barbus sharpeyi nationallicence Cortisol nationallicence Testosterone nationallicence Progesterone nationallicence Estradiol- 17β nationallicence Ovaprim nationallicence Mohammadian Takavar Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran aut Malekpouri Pedram Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran aut Zare Mojtaba Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran aut Zainodini Mohammad Department of Basic Science, Chabahar Maritime and Marine Science University, Chabahar, Iran aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1475-1489 0920-1742 41:6<1475 2015 41 10695 https://doi.org/10.1007/s10695-015-0100-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0100-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohammadian Takavar Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran aut NATIONALLICENCE 700 1- Malekpouri Pedram Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran aut NATIONALLICENCE 700 1- Zare Mojtaba Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran aut NATIONALLICENCE 700 1- Zainodini Mohammad Department of Basic Science, Chabahar Maritime and Marine Science University, Chabahar, Iran aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1475-1489 0920-1742 41:6<1475 2015 41 10695