caa a22 4500 606229760 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0106-1 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0106-1 Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I. furcatus [Elektronische Daten] [Mei Shang, Baofeng Su, Elizabeth Lipke, Dayan Perera, Chao Li, Zhenkui Qin, Yun Li, David Dunn, Sehriban Cek, Eric Peatman, Rex Dunham] Testicular germ cells of channel catfish, Ictalurus punctatus, and blue catfish, I. furcatus were separated into four layers with Percoll density gradient centrifugation, containing different cell types (40% in the first layer were spermatogonial stem cells, SSCs). Expression of seventeen genes was analyzed for cells from different layers by real-time quantitative PCR. Pfkfb4, Urod, Plzf, Integrin6, IntegrinV, Thy1 and Cdh1 genes showed the same expression change pattern in both channel and blue catfish as these genes were down-regulated in the spermatocytes and even more so in spermatids. Plzf and Integrin6 had especially high expression in SSCs and can be used as SSCs specific markers. Sox2 gene was up-regulated in spermatocytes and even more highly up-regulated in spermatids, which indicated it could be a spermatid marker.In contrast to channel catfish,Id4,Smad5andPrdm14geneexpressions were strongly down-regulated in spermatocyte cells, but up-regulated in spermatid cells in blue catfish. Smad5 gene was down-regulated in spermatocytes, but up-regulated in both spermatogonia and spermatids, allowing identification as a marker for spermatocytesin blue catfish. Oct4, Id4, Gfrα2, Pum2 and Prdm14 genes showed different expression patterns in the testicular germ cells of channel and blue catfish. This may be a partial explanation to the differing responses of channel catfish and blue catfish to induced spawning technologies. The SSCs specific markers can be used for further SSCs labeling, which can increase the SSCs sorting efficiency and be applied in various studies involving SSCs and other germ cells. Springer Science+Business Media Dordrecht, 2015 Catfish nationallicence Spermatogonia stem cell nationallicence Density gradient centrifugation nationallicence Gene expression nationallicence Marker gene nationallicence SSCs : Spermatogonial stem cells nationallicence PGCs : Primordial germ cells nationallicence TL : Total length nationallicence qPCR : Real-time quantitative PCR nationallicence Shang Mei School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut Su Baofeng School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut Lipke Elizabeth Department of Chemical Engineering, Auburn University, 36849, Auburn, AL, USA aut Perera Dayan School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut Li Chao School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut Qin Zhenkui School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut Li Yun School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut Dunn David Department of Chemical Engineering, Auburn University, 36849, Auburn, AL, USA aut Cek Sehriban School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut Peatman Eric School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut Dunham Rex School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1545-1556 0920-1742 41:6<1545 2015 41 10695 https://doi.org/10.1007/s10695-015-0106-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0106-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shang Mei School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Su Baofeng School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Lipke Elizabeth Department of Chemical Engineering, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Perera Dayan School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Li Chao School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Qin Zhenkui School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Li Yun School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Dunn David Department of Chemical Engineering, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Cek Sehriban School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Peatman Eric School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Dunham Rex School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1545-1556 0920-1742 41:6<1545 2015 41 10695