caa a22 4500 606229779 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0092-3 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0092-3 Effects on the metabolism, growth, digestive capacity and osmoregulation of juvenile of Sub-Antarctic Notothenioid fish Eleginops maclovinus acclimated at different salinities [Elektronische Daten] [L. Vargas-Chacoff, E. Saavedra, R. Oyarzún, E. Martínez-Montaño, J. Pontigo, A. Yáñez, I. Ruiz-Jarabo, J. Mancera, E. Ortiz, C. Bertrán] In this study we assessed the influence of three different environmental salinities (5, 15 and 31psu during 90days) on growth, osmoregulation, energy metabolism and digestive capacity in juveniles of the Notothenioid fish Eleginops maclovinus. At the end of experimental time samples of plasma, liver, gill, intestine, kidney, skeletal muscle, stomach and pyloric caeca were obtained. Growth, weight gain, hepatosomatic index and specific growth rate increased at 15 and 31psu and were lower at 5psu salinity. Gill Na+, K+-ATPase (NKA) activity presented a "U-shaped” relationship respect to salinity, with its minimum rates at 15psu, while this activity correlated negatively with salinity at both anterior and posterior intestinal portions. No significant changes in NKA activity were observed in kidney or mid intestine. Large changes in plasma, metabolite levels and enzymatic activities related to energy metabolism in liver, gill, intestine, kidney and muscle were generally found in the groups exposed to 5 and 31psu compared to the 15psu group. Only the pepsin activity (digestive enzymes) assessed enhanced with environmental salinity, while pyloric caeca trypsin/chymotrypsin ratio decreased. This study suggests that juvenile of E. maclovinus presents greater growth near its iso-osmotic point (15psu) and hyperosmotic environment (31psu). Acclimation to low salinity increased the osmoregulatory expenditure as seen by the gill and anterior intestine results, while at high salinity, branchial osmoregulatory activity was also enhanced. This requires the mobilization of lipid stores and amino acids, thereby holding the growth of fish back. The subsequent reallocation of energy sources was not sufficient to maintain the growth rate of fish exposed to 5psu. Thus, E. maclovinus juveniles present better growth efficiencies in salinities above the iso-osmotic point and hyperosmotic environment of this species, showing their best performance at 15psu as seen by the main osmoregulatory and energy metabolism enzymatic activities. Springer Science+Business Media Dordrecht, 2015 Eleginops maclovinus nationallicence Euryhaline fish nationallicence Metabolism nationallicence Growth nationallicence Osmoregulation nationallicence Vargas-Chacoff L. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut Saavedra E. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut Oyarzún R. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut Martínez-Montaño E. Centro de Investigación y Desarrollo (CIEN Austral) CONICYT Regional R10C1002, Universidad Austral de Chile, Av. Los Pinos s/n, Balneario Pelluco, Puerto Montt, Chile aut Pontigo J. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut Yáñez A. Instituto de Bioquímica y Microbiología, Interdisciplinary Center for Aquaculture Research (FONDAP-INCAR), Universidad Austral de Chile, Valdivia, Chile aut Ruiz-Jarabo I. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, Cádiz, Spain aut Mancera J. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, Cádiz, Spain aut Ortiz E. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut Bertrán C. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1369-1381 0920-1742 41:6<1369 2015 41 10695 https://doi.org/10.1007/s10695-015-0092-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0092-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vargas-Chacoff L. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut NATIONALLICENCE 700 1- Saavedra E. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut NATIONALLICENCE 700 1- Oyarzún R. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut NATIONALLICENCE 700 1- Martínez-Montaño E. Centro de Investigación y Desarrollo (CIEN Austral) CONICYT Regional R10C1002, Universidad Austral de Chile, Av. Los Pinos s/n, Balneario Pelluco, Puerto Montt, Chile aut NATIONALLICENCE 700 1- Pontigo J. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut NATIONALLICENCE 700 1- Yáñez A. Instituto de Bioquímica y Microbiología, Interdisciplinary Center for Aquaculture Research (FONDAP-INCAR), Universidad Austral de Chile, Valdivia, Chile aut NATIONALLICENCE 700 1- Ruiz-Jarabo I. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, Cádiz, Spain aut NATIONALLICENCE 700 1- Mancera J. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, Cádiz, Spain aut NATIONALLICENCE 700 1- Ortiz E. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut NATIONALLICENCE 700 1- Bertrán C. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1369-1381 0920-1742 41:6<1369 2015 41 10695