caa a22 4500 606229787 CHVBK 20210128101210.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0094-1 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0094-1 Ontogeny and kinetics of carnitine palmitoyltransferase I in hepatopancreas and skeletal muscle of grass carp ( Ctenopharyngodon idella ) [Elektronische Daten] [Wei Hu, Zhi Luo, Kang-Sen Mai, Cai-Xia Liu, Jia-Lang Zheng] The ontogeny and kinetics of carnitine palmitoyltransferase I (CPT I) were investigated in hepatopancreas and muscle throughout four developmental stages (newly hatched larvae, 1-month-old juvenile, 3-month-old, and 6-month-old, respectively) of grass carp Ctenopharyngodon idella. In hepatopancreas, the maximal velocity (V max) significantly increased from hatching to 1-month-old grass carp and then gradually declined at 6-month-old grass carp. In muscle, CPT I activity was the highest at 1-month-old grass carp, nearly twofold higher than that at hatching (P<0.05). The Michaelis constant (K m) value was also the highest for 1-month-old in both tested tissues. Carnitine concentrations (FC, AC and TC) were the lowest for 3-month-old grass carp and remained relatively constant in both tissues from fish under the other developmental stages. The FC concentration in hepatopancreas and muscle at four developmental stages were less than the respective K m, indicating that grass carp required supplemental carnitine in their food to ensure that CPT I activity was not constrained by carnitine availability. Springer Science+Business Media Dordrecht, 2015 Ctenopharyngodon idella nationallicence Carnitine nationallicence CPT I nationallicence Kinetics nationallicence Development nationallicence AC : Acyl carnitine nationallicence TC : Total carnitine nationallicence FC : Free carnitine nationallicence ANOVA : One-way analysis of variance nationallicence CPT : Carnitine palmitoyltransferase nationallicence DTNB : 5, 5′-dithio-bis-(2-nitrobenzoic acid) nationallicence K m : Michaelis-Menten constants nationallicence MS 222 : Tricaine methanesulfonate nationallicence SEM : Standard error of mean nationallicence V max : Maximal reaction velocity nationallicence Hu Wei Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Luo Zhi Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Mai Kang-Sen College of Fisheries, Ocean University of China, 266003, Qingdao, People's Republic of China aut Liu Cai-Xia Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Zheng Jia-Lang Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1393-1401 0920-1742 41:6<1393 2015 41 10695 https://doi.org/10.1007/s10695-015-0094-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0094-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Wei Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Luo Zhi Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Mai Kang-Sen College of Fisheries, Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Liu Cai-Xia Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Jia-Lang Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1393-1401 0920-1742 41:6<1393 2015 41 10695