caa a22 4500 606229817 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0101-6 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0101-6 Carbonic anhydrase 2-like and Na+-K+-ATPase α gene expression in medaka ( Oryzias latipes ) under carbonate alkalinity stress [Elektronische Daten] [Zongli Yao, Qifang Lai, Zhuoran Hao, Ling Chen, Tingting Lin, Kai Zhou, Hui Wang] High carbonate alkalinity is one of the major stress factors for living organisms in saline-alkaline water areas. Acute and chronic effects of carbonate alkalinity on expression of two genes, carbonic anhydrase 2-like (CA2-like) and Na+-K+-ATPase α subunit (NKA-α) mRNA in medaka (Oryzias latipes) were evaluated to better understand the responses important for coping with a carbonate alkalinity stress. In the acute exposure experiment, the expression of CA2-like and NKA-α mRNA in the gill and kidney of medaka were examined from 0h to 7days exposed to 30.4mM carbonate alkalinity water. Exposure to high carbonate alkalinity resulted in a transitory alkalosis, followed by a transient increase in gill and kidney CA2-like and NKA-α mRNA expression. In the chronic exposure experiment, the expression of these two genes was examined in the gill and kidney at 50days post-exposure to six different carbonate alkalinity concentrations ranging from 1.5 to 30.4mM. Gill and kidney CA2-like mRNA levels in 30.4mM were approximately 10 and 30 times higher than that of the control (1.5mM), respectively. Less differences were found in NKA-α expression in the 50-days exposure. The results indicate that when transferred to high carbonate alkalinity water, a transitory alkalosis may occur in medaka, followed by compensatory acid-base and ion regulatory responses. Thus, CA2-like and NKA-α are at least two of the important factors that contribute to the regulation of alkalinity stress. Springer Science+Business Media Dordrecht, 2015 Carbonic anhydrase nationallicence $${\hbox {Na}}^{+}$$ Na + - $${\hbox {K}}^{+}$$ K + -ATPase nationallicence Alkalinity stress nationallicence Oryzias latipes nationallicence Quantitative real-time PCR nationallicence Yao Zongli Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut Lai Qifang Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut Hao Zhuoran Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut Chen Ling State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, China aut Lin Tingting Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut Zhou Kai Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut Wang Hui Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1491-1500 0920-1742 41:6<1491 2015 41 10695 https://doi.org/10.1007/s10695-015-0101-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0101-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yao Zongli Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut NATIONALLICENCE 700 1- Lai Qifang Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut NATIONALLICENCE 700 1- Hao Zhuoran Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut NATIONALLICENCE 700 1- Chen Ling State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, China aut NATIONALLICENCE 700 1- Lin Tingting Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut NATIONALLICENCE 700 1- Zhou Kai Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut NATIONALLICENCE 700 1- Wang Hui Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 20090, Shanghai, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1491-1500 0920-1742 41:6<1491 2015 41 10695